Issue 8, 2020

Study on antibacterial properties and cytocompatibility of EPL coated 3D printed PCL/HA composite scaffolds

Abstract

Biomaterial scaffolds play a critical role in bone tissue engineering. Moreover, 3D printing technology has enormous advantage in the manufacture of bioengineering scaffolds for patient-specific bone defect treatments. In order to provide an aseptic environment for bone regeneration, ε-poly-L-lysine (EPL), an antimicrobic cationic polypeptide, was used for surface modification of 3D printed polycaprolactone/hydroxyapatite (PCL/HA) scaffolds which were fabricated by fused deposition modeling (FDM) technology. The scaffold morphology and micro-structure were characterized by scanning electron microscopy (SEM), X-ray diffraction (XRD) and transform infrared spectroscopy (FT-IR). The release profile surface roughness, open porosity, and mechanical properties of the scaffolds were evaluated. Cell adhesion, proliferation, differentiation potential and antibacterial properties were also examined. As a result, 3D printed PCL/HA scaffolds with interconnected pores showed a slightly rough surface and improved mechanical properties due to adding hydroxyapatite (HA) particles. After being modified by EPL, favorable biocompatibility and osteoconductivity of ε-poly-L-lysine/polycaprolactone/hydroxyapatite (EPL/PCL/HA) scaffolds were observed. Moreover, antibacterial activity of the EPL/PCL/HA scaffolds was apparent. As a consequence, the EPL/PCL/HA scaffolds had great potential for bone regeneration and prevention of infections. This would yield a patient-specific bioactive and antibacterial composite scaffold for advanced bone tissue engineering applications.

Graphical abstract: Study on antibacterial properties and cytocompatibility of EPL coated 3D printed PCL/HA composite scaffolds

  • This article is part of the themed collection: Biomaterials

Article information

Article type
Paper
Submitted
07 dec 2019
Accepted
19 jan 2020
First published
29 jan 2020
This article is Open Access
Creative Commons BY-NC license

RSC Adv., 2020,10, 4805-4816

Study on antibacterial properties and cytocompatibility of EPL coated 3D printed PCL/HA composite scaffolds

L. Tian, Z. Zhang, B. Tian, X. Zhang and N. Wang, RSC Adv., 2020, 10, 4805 DOI: 10.1039/C9RA10275B

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