Issue 61, 2018

ESIPT-based fluorescence probe for the rapid detection of hypochlorite (HOCl/ClO)

Abstract

ESIPT-based fluorescence probes are emerging as an attractive tool for the detection of biologically relevant analytes owing to their unique photophysical properties. In this work, we have developed an ESIPT-based fluorescence probe (TCBT-OMe) for the detection of HClO/ClO through the attachment of a bioorthogonal dimethylthiocarbamate linker. TCBT-OMe was shown to rapidly detect HClO/ClO (<10 s) at biologically relevant concentrations (LoD = 0.16 nM) and have an excellent selectivity towards others ROS/RNS and amino acids. Therefore, TCBT-OMe was tested in live cells and was successfully shown to be able to detect endogenous and exogenous HClO/ClO in HeLa cells. Additionally, TCBT-OMe acts as a dual input logic gate for Hg2+ and H2O2. Interestingly, Hg2+ alone gradually causes a fluorescence response but requires >30 min to produce a fluorescence response. Test strips containing TCBT-OMe were prepared and were demonstrated as an effective way to detect HClO/ClO in water. Furthermore, TCBT-OMe was shown to detect exogenously added HClO/ClO in three different water samples with little interference thus demonstrating the effectiveness as a method for the detection of HClO/ClO in drinking water samples.

Graphical abstract: ESIPT-based fluorescence probe for the rapid detection of hypochlorite (HOCl/ClO−)

Supplementary files

Article information

Article type
Communication
Submitted
08 maj 2018
Accepted
28 jun 2018
First published
04 jul 2018
This article is Open Access
Creative Commons BY license

Chem. Commun., 2018,54, 8522-8525

ESIPT-based fluorescence probe for the rapid detection of hypochlorite (HOCl/ClO)

L. Wu, Q. Yang, L. Liu, A. C. Sedgwick, A. J. Cresswell, S. D. Bull, C. Huang and T. D. James, Chem. Commun., 2018, 54, 8522 DOI: 10.1039/C8CC03717E

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