Issue 9, 2020

Single-cell analysis by use of ICP-MS

Abstract

This tutorial review article is highlighting the fundamentals, instrumentation, and most recent trends of single-cell analysis by use of inductively coupled plasma-mass spectrometry (ICP-MS). It is shown that metals and hetero-elements being intrinsically present in cells, taken up by cells (for instance engineered metallic nanoparticles) or binding to a cell can be detected qualitatively by existing ICP-MS technologies on a single cell level. Adding a quantitative dimension to single-cell analysis by (laser ablation-) ICP-MS requires dedicated calibration and validation strategies, which are currently being established and are being critically discussed. In a tutorial part, the ICP-MS instruments, the measurement conditions, and the sample introduction and preparation techniques are introduced. The application section focuses on the state-of-the-art of single-cell analysis in suspension, using laser ablation or (imaging) mass cytometry. Finally, future trends are critically assessed.

Graphical abstract: Single-cell analysis by use of ICP-MS

Article information

Article type
Tutorial Review
Submitted
27 apr 2020
Accepted
17 aug 2020
First published
24 aug 2020

J. Anal. At. Spectrom., 2020,35, 1784-1813

Single-cell analysis by use of ICP-MS

S. Theiner, K. Loehr, G. Koellensperger, L. Mueller and N. Jakubowski, J. Anal. At. Spectrom., 2020, 35, 1784 DOI: 10.1039/D0JA00194E

To request permission to reproduce material from this article, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.

Social activity

Spotlight

Advertisements