Issue 16, 2018

Interfacial electrostatics of poly(vinylamine hydrochloride), poly(diallyldimethylammonium chloride), poly-l-lysine, and poly-l-arginine interacting with lipid bilayers

Abstract

Charge densities of cationic polymers adsorbed to lipid bilayers are estimated from second harmonic generation (SHG) spectroscopy and quartz crystal microbalance with dissipation monitoring (QCM-D) measurements. The systems surveyed included poly(vinylamine hydrochloride) (PVAm), poly(diallyldimethylammonium chloride) (PDADMAC), poly-L-lysine (PLL), and poly-L-arginine (PLR), as well as polyalcohol controls. Upon accounting for the number of positive charges associated with each polyelectrolyte, the binding constants and apparent free energies of adsorption as estimated from SHG data are comparable despite differences in molecular masses and molecular structure, with ΔGads values of −61 ± 2, −58 ± 2, −57 ± 1, −52 ± 2, −52 ± 1 kJ mol−1 for PDADMAC400, PDADMAC100, PVAm, PLL, and PLR, respectively. Moreover, we find charge densities for polymer adlayers of approximately 0.3 C m−2 for poly(diallyldimethylammonium chloride) while those of poly(vinylamine) hydrochloride, poly-L-lysine, and poly-L-arginine are approximately 0.2 C m−2. Time-dependent studies indicate that polycation adsorption to supported lipid bilayers is only partially reversible for most of the polymers explored. Poly(diallyldimethylammonium chloride) does not demonstrate reversible binding even over long timescales (>8 hours).

Graphical abstract: Interfacial electrostatics of poly(vinylamine hydrochloride), poly(diallyldimethylammonium chloride), poly-l-lysine, and poly-l-arginine interacting with lipid bilayers

Supplementary files

Article information

Article type
Paper
Submitted
30 okt 2017
Accepted
19 feb 2018
First published
19 feb 2018

Phys. Chem. Chem. Phys., 2018,20, 10846-10856

Interfacial electrostatics of poly(vinylamine hydrochloride), poly(diallyldimethylammonium chloride), poly-L-lysine, and poly-L-arginine interacting with lipid bilayers

A. C. McGeachy, N. Dalchand, E. R. Caudill, T. Li, M. Doğangün, L. L. Olenick, H. Chang, J. A. Pedersen and F. M. Geiger, Phys. Chem. Chem. Phys., 2018, 20, 10846 DOI: 10.1039/C7CP07353D

To request permission to reproduce material from this article, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.

Social activity

Spotlight

Advertisements