Issue 23, 2025
From the journal:

Chemical Communications

The time course of in vivo cellular responses to LNPs†

David Loughrey, ORCID logo ‡*a   Kalina Paunovska,‡a   Elisa Schrader Echeverri,a   Karen E. Tiegreena  and  James E. Dahlman ORCID logo *a  

* Corresponding authors

a Wallace H. Coulter Department of Biomedical Engineering, Georgia Institute of Technology, Atlanta, GA, USA
E-mail: david.loughrey@gmail.com, james.dahlman@bme.gatech.edu

Abstract

Cells actively respond to drug delivery systems. However, the time course of cellular responses to lipid nanoparticles (LNPs) remains unclear. Here we characterized the transcriptomic response to LNPs carrying mRNA at different timepoints in vivo. Exposure to LNPs altered the expression of signaling pathways including endocytosis and lysosomal pathways as soon as one hour after administration. These pathways returned to their baseline state by 24 hours. Our data are consistent with the hypothesis that cells actively yet transiently respond to LNPs.

Graphical abstract: The time course of in vivo cellular responses to LNPs

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Article information

DOI
https://doi.org/10.1039/D4CC06659F
Article type
Communication
Submitted
20 pro 2024
Accepted
05 úno 2025
First published
11 úno 2025
This article is Open Access
Creative Commons BY license

Chem. Commun., 2025,61, 4535-4538

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The time course of in vivo cellular responses to LNPs

D. Loughrey, K. Paunovska, E. S. Echeverri, K. E. Tiegreen and J. E. Dahlman, Chem. Commun., 2025, 61, 4535 DOI: 10.1039/D4CC06659F

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