Enhancing protein stability under stress: osmolyte-based deep eutectic solvents as a biocompatible and robust stabilizing medium for lysozyme under heat and cold shock

Anja Damjanović, Marijan Logarušić, Lidija-Marija Tumir, Thanos Andreou, Marina Cvjetko Bubalo and Ivana Radojčić Redovniković

Phys. Chem. Chem. Phys., 2024, 26, 21040
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Towards the sustainable extraction and purification of non-animal proteins from biomass using alternative solvents

Bojan Kopilovic, Ana I. Valente, Ana M. Ferreira, Mafalda R. Almeida, Ana P. M. Tavares, Mara G. Freire and João A. P. Coutinho

RSC Sustainability, 2023, 1, 1314
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Replacing all petroleum-based chemical products with natural biomass-based chemical products: a tutorial review

Ryohei Mori

RSC Sustainability, 2023, 1, 179
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The attenuating ability of deep eutectic solvents towards the carboxylated multiwalled carbon nanotubes induced denatured β-lactoglobulin structure

Niketa Yadav, Sanjay Mor and Pannuru Venkatesu

Phys. Chem. Chem. Phys., 2023, 25, 20519
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Natural multi-osmolyte cocktails form deep eutectic systems of unprecedented complexity: discovery, affordances and perspectives

Marina Cvjetko Bubalo, Thanos Andreou, Manuela Panić, Mia Radović, Kristina Radošević and Ivana Radojčić Redovniković

Green Chem., 2023, 25, 3398
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Essential dynamics of ubiquitin in water and in a natural deep eutectic solvent

Inês Gomes, Gabriel. F. Martins and Nuno Galamba

Phys. Chem. Chem. Phys., 2024, 26, 18244
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Unravelling the influence of choline chloride-based deep eutectic solvents on lysozyme: a comparative study of fructose and formic acid donors

Ntebogeng Mqoni, Sheldon Sookai, Ajit Kumar, Lebogang Katata-Seru, Faruq Mohammad, Pannuru Venkatesu, Indra Bahadur and Ahmed Abdullah Soleiman

RSC Adv., 2025, 15, 25483
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Hydration in Deep Eutectic Solvents Induces Non-monotonic Changes in the Conformation and Stability of Proteins

Adrian Sanchez-Fernandez, Medina Basic, Jenny Xiang, Sylvain Prevost, Andrew J. Jackson and Cedric Dicko

J. Am. Chem. Soc., 2022, 144, 23657
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Assessing the compatibility of choline-based deep eutectic solvents for the structural stability and activity of cellulase: Enzyme sustain at high temperature

Niketa Yadav, Deepak Chahar, Meena Bisht and Pannuru Venkatesu

International Journal of Biological Macromolecules, 2023, 249, 125988
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Aktivitätssteigerung von Hydrogenase zur photokatalytischen Wasserstofferzeugung an Luft mittels Lösemitteltuning

Michael G. Allan, Thomas Pichon, Jade A. McCune, Christine Cavazza, Alan Le Goff and Moritz F. Kühnel

Angewandte Chemie, 2023, 135
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Eutectogels as Promising Materials in Biocatalysis

Krishan Kumar, Marcelo Calderón, Ana Beloqui and Matias L. Picchio

ChemCatChem, 2024, 16
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Thermal stability and refolding of Hen Egg-White Lysozyme in aqueous Deep Eutectic Solvent solutions

Samal Kaumbekova and Dhawal Shah

Journal of Molecular Liquids, 2023, 389, 122939
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Protein stability in a natural deep eutectic solvent: Preferential hydration or solvent slaving?

Inês Gomes and Nuno Galamba

The Journal of Chemical Physics, 2023, 159
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Valorization of biomass for food protein via deep eutectic solvent extraction: Understanding the extraction mechanism and impact on protein structure and properties

Mohamad Ariff Hanafi, Farooq Anwar and Nazamid Saari

Food Frontiers, 2024, 5, 1265
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Steady and cyclic recovery of potato protein from starch waste via tailored deep eutectic solvent system

Wenyu Zheng, Zhicheng Niu, Dan Yuan, Jiale Zhang, Ningqiao Kong, Mouming Zhao and Feibai Zhou

Chemical Engineering Journal, 2025, 513, 162975
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Site-specific water dynamics drives protein stability in hydrated deep eutectic solvents

Tanmoy Khan, Kuntal Debnath, Kuldeep Singh Negi and Pratik Sen

Biophysical Journal, 2025, 124, 4255
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Cyclic Peptide Nanotubes in Deep Eutectic Solvents: Insights into Stability, Hydration, and Thermal Effects

Rimjhim Moral and Sandip Paul

J. Phys. Chem. B, 2025, 129, 7861
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Stabilization of Non-Native Folds and Programmable Protein Gelation in Compositionally Designed Deep Eutectic Solvents

Adrian Sanchez-Fernandez, Jia-Fei Poon, Anna Elizabeth Leung, Sylvain François Prévost and Cedric Dicko

ACS Nano, 2024, 18, 18314
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Modulating protein unfolding and refolding via the synergistic association of an anionic and a nonionic surfactant

Johanna Hjalte, Carl Diehl, Anna E. Leung, Jia-Fei Poon, Lionel Porcar, Rob Dalgliesh, Helen Sjögren, Marie Wahlgren and Adrian Sanchez-Fernandez

Journal of Colloid and Interface Science, 2024, 672, 244
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A Vibrant Accelerating Strategic Solvent System for Sustainability and Functionality for Proteins: Unvarying Structure of Stem Bromelain in Hybrid Ionic Fluids

Srinuvasu Nakka, Anamika Sindhu, Deepak Chahar, Meena Bisht, Nagaraju Devunuri and Pannuru Venkatesu

ACS Sustainable Chem. Eng., 2023, 11, 6395
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Modulation of surfactant self-assembly in deep eutectic solvents and its relevance to drug delivery-A review

M. Basu, Puthusserickal A. Hassan and Sandeep B. Shelar

Journal of Molecular Liquids, 2023, 375, 121301
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Preservation of biological systems and materials using deep eutectic solvents: Pinnacles and pitfalls

Heedayah H. Azouz and Maan Hayyan

Separation and Purification Technology, 2026, 382, 135584
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Exploring the potential of deep eutectic solvents in pharmaceuticals: Challenges and opportunities

Priyanka A. Shah, Vishwajit Chavda, Darshna Hirpara, Vinay S. Sharma, Pranav S. Shrivastav and Sanjeev Kumar

Journal of Molecular Liquids, 2023, 390, 123171
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Intermolecular Interactions Governing Extraction Efficiency of Polycyclic Thiophenes by Deep Eutectic Solvents

Miyuru M. Wellalage, Maleesha T. Fernando, Emily M. Molino, Madeline Schultz, Nwanne D. Banor, Neil A. Ellis and Daniel A. Thomas

Energy Fuels, 2025, 39, 24065
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Scattering approaches to unravel protein solution behaviors in ionic liquids and deep eutectic solvents: From basic principles to recent developments

Qi Han, Nathalia V.P. Veríssimo, Saffron J. Bryant, Andrew V. Martin, Yuhong Huang, Jorge F.B. Pereira, Valéria C. Santos-Ebinuma, Jiali Zhai, Gary Bryant, Calum J. Drummond and Tamar L. Greaves

Advances in Colloid and Interface Science, 2024, 331, 103242
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Assessing Deep Eutectic Solvent Efficiency by Optimizing Soybean Protein Extraction

Chaima Neji, Arjun Muthu, Diána Ungai, Emese Seres, Éva Domokos-Szabolcsy, József Prokisch, Endre Máthé and Péter Sipos

Food Bioprocess Technol, 2025, 18, 9423
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Fabrication and Properties of Anti-freezing Gelatin Hydrogels Based on a Deep Eutectic Solvent

Runpeng Liu, Congde Qiao, Qinze Liu, Libin Liu and Jinshui Yao

ACS Appl. Polym. Mater., 2023, 5, 4546
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Improved activity and thermostability of glycosylating biocatalysts acting in neat natural deep eutectic solvents: Pushing boundaries of enzymes

K. Bautista-Rangel, L.F. Losoya-Uribe, M. Rodríguez-Gonzalez, G. Saab-Rincón, A. López-Munguía and E. Castillo

International Journal of Biological Macromolecules, 2025, 318, 145012
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Hydration-mediated stabilization and anti-aggregation of soy and whey protein isolates, insulin, and collagen in hydrophilic deep eutectic solvents

Jiawen Chen, Keting Chen, Tianqi Liu, Mi Wang, Xiangli Liu, Jichuan Zhang and Jiaheng Zhang

Journal of Colloid and Interface Science, 2026, 707, 139683
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Augmenting the Performance of Hydrogenase for Aerobic Photocatalytic Hydrogen Evolution via Solvent Tuning

Michael G. Allan, Thomas Pichon, Jade A. McCune, Christine Cavazza, Alan Le Goff and Moritz F. Kühnel

Angew Chem Int Ed, 2023, 62
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Inhibiting insulin aggregation by chaperone-like green cholinium-based DESs as additives

Elnaz Hosseini, Payam Arghavani, Morteza Torabi, Mohammad Ali Zolfigol, Ali Sharifi, Mojtaba Mirzaei, Faezeh Moosavi-Movahedi, Ahmad Amiri, Reza Yousefi, Mehran Habibi-Rezaei and Ali Akbar Moosavi-Movahedi

Journal of Molecular Liquids, 2024, 407, 125274
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Unlocking the full compositional control of hydrophilic and hydrophobic deep eutectic solvents over protein structure and stability

Adrian Sanchez-Fernandez, Jake H. Nicholson, Susana M. Meza Huaman, Claudia Almuzara Romero, Jia-Fei Poon, Sylvain Prevost and Alex P. S. Brogan

Commun Chem, 2025, 8
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A temperature-triggered phase separation system for stabilizing bioactive protein structure and function

Yanan Wei, Zhijie Li, Fan Ni, Fei Zhang, Qiang Zhang and Zejun Wang

Talanta, 2026, 306, 129675
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Production of Xylitol and Ethanol from Agricultural Wastes and Biotransformation of Phenylacetylcarbinol in Deep Eutectic Solvent

Su Lwin Htike, Julaluk Khemacheewakul, Charin Techapun, Yuthana Phimolsiripol, Pornchai Rachtanapun, Suphat Phongthai, Worasit Tochampa, Siraphat Taesuwan, Kittisak Jantanasakulwong, Kritsadaporn Porninta, Sumeth Sommanee, Chatchadaporn Mahakuntha, Juan Feng, Anbarasu Kumar, Xinshu Zhuang, Wen Wang, Wei Qi, Rojarej Nunta and Noppol Leksawasdi

Agriculture, 2024, 14, 2043
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Deep eutectic solvents for protein extraction: mechanisms, performance, and green metrics

Joel Ching Jue Wong, Ianatul Khoiroh, Suyin Gan and Anna K. Croft

Journal of Molecular Liquids, 2025, 438, 128636
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Dissolving Amyloid Fibrils with Natural Deep Eutectic Solvents: Citric Acid−Glycerol Achieves Superior Solubilization and Partial Protein Refolding

Sora Hirano, Tatsuya Oshima, Asuka Inada and Toshihiro Tsuruda

ACS Appl. Bio Mater., 2026
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Unveiling the Influence of Hydrated Deep Eutectic Solvents on the Dynamics of Water–Soluble Proteins

Gianluca Parisse, Daniele Narzi, Benny Danilo Belviso, Vito Capriati, Rocco Caliandro, Massimo Trotta and Leonardo Guidoni

J. Phys. Chem. B, 2023, 127, 6487
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Completely Inorganic Deep Eutectic Solvents for Efficient and Recyclable Liquid–Liquid Interface Catalysis

Lixian Xu, Jie Yin, Jing He, Hongping Li, Linhua Zhu, Hailong Ning, Kecheng Jie, Wenshuai Zhu, Huaming Li, Sheng Dai and Wei Jiang

Advanced Materials, 2024, 36
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Green Protein Extraction from Hazelnut Press Cake: Yield, Efficiency, and Secondary Structure Analysis

Bruna Anzà, Silvia Fraterrigo Garofalo, Alessandro Lapolla and Debora Fino

Separations, 2025, 12, 63
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Lysozyme stability in various deep eutectic solvents using molecular dynamics simulations

Akshatha Hebbar, Poulumi Dey and Anoop Kishore Vatti

Journal of Biomolecular Structure and Dynamics, 2024, 42, 13325
DOI: 10.1080/07391102.2023.2275178