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Self-primer exponential Cas12a tandem reaction-amplified and [Cu(tz)] nanosheets based neoteric biosensing platform for quantifying multiple mTBI-related miRNAs
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Recent advances in isothermal amplification techniques coupled with clustered regularly interspaced short palindromic repeat/Cas systems
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Engineering Anti‐CRISPR Proteins to Create CRISPR‐Cas Protein Switches for Activatable Genome Editing and Viral Protease Detection
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Advancing CRISPR-Based Solutions for COVID-19 Diagnosis and Therapeutics
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Cells, 2024, 13, 1794
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Temperature-boosted PAM-less activation of CRISPR-Cas12a combined with selective inhibitors enhances detection of SNVs with VAFs below 0.01%
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Integrating CRISPR-Cas12a with catalytic hairpin assembly as a logic gate biosensing platform for the detection of polychlorinated biphenyls in water samples
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Science of The Total Environment, 2023, 881, 163465
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Bioreceptors as the key components for electrochemical biosensing in medicine
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Multiplexed lateral flow assay integrated with orthogonal CRISPR-Cas system for SARS-CoV-2 detection
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Sensors and Actuators B: Chemical, 2022, 371, 132537
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CRISPR-initiated exponential amplification on fluorescently-barcoded microspheres for deep learning-assisted multiplexed HPV detection
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Point‐of‐Care Diagnosis of Respiratory Viruses at Single‐Nucleotide Resolution with an Autocatalytic Rolling Circle Amplification System
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CRISPR/Cas12a-based MUSCA-PEC strategy for HSV-1 assay
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CRISPR/Cas systems accelerating the development of aptasensors
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Research Progress of CRISPR-Cas System in Medical Diagnosis
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Research Progress of CRISPR Technology for Accurate Diagnosis and Treatment in Oncology
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Rapid and sensitive detection of bovine Theileria annulata parasite based on ERA-CRISPR/Cas12a technology
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Sensitive CRISPR-Cas12a-Assisted Immunoassay for Small Molecule Detection in Homogeneous Solution
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An ultrasensitive sensing strategy based on CRISPR/Cas13a and T7 RNA polymerase amplification for detection of extracellular vesicles
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PAM‐Engineered Toehold Switches as Input‐Responsive Activators of CRISPR‐Cas12a for Sensing Applications
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Ultrasensitive RNase H activity detection using the transcription-based hybrid probe and CRISPR/cas12a signal amplifier
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Portable biosensors based on the CRISPR/Cas system for detection of pathogen bacteria: Up-to-date technology and future prospects
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Microchemical Journal, 2023, 194, 109268
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Dual CRISPR/Cas13a Cascade Strand Displacement-Triggered Transcription for Point-of-Care Detection of Plasmodium in Asymptomatic Malaria
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Recent advances and challenges of the use of the CRISPR/Cas system as a non-nucleic acid molecular diagnostic
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Recent Advances in CRISPR/Cas-Based Biosensors for Protein Detection
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CRISPR-based dual-aptamer proximity ligation coupled hybridization chain reaction for precise detection of tumor extracellular vesicles and cancer diagnosis
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CRISPR/Cas12a‐Enabled Multiplex Biosensing Strategy Via an Affordable and Visual Nylon Membrane Readout
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Advanced Science, 2023, 10
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Developing a Versatile Arsenal: Novel Antimicrobials as Offensive Tools Against Pathogenic Bacteria
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CRISPR-Cas System: A New Dawn to Combat Antibiotic Resistance
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PAM-Independent CRISPR-Cas12a System for Specific Assays of Single Nucleotide Variants
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JACS Au, 2025, 5, 1392
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Rapid Amplification and Detection of Single‐Stranded Nucleic Acids for Point‐of‐Care Diagnosis
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Small Methods, 2025, 9
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Programmable DNA-Fueled Electrochemical Analysis of Lung Cancer Exosomes
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A switchable Cas12a enabling CRISPR-based direct histone deacetylase activity detection
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Biosensors and Bioelectronics, 2022, 213, 114468
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A proximity induced strand displacement amplification and CRISPR/Cas12a-based SERS assay for ultrasensitive detection of dengue virus
Jiayin Zhang, Weipeng Zhang, Jieyu Yan, Yirui Zhang, Jie Zheng, Yiyi Liu, Ning Gu and Kun Han
Biosensors and Bioelectronics, 2026, 304, 118651
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Recent Advances in Microfluidics for the Early Detection of Plant Diseases in Vegetables, Fruits, and Grains Caused by Bacteria, Fungi, and Viruses
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A PAM-free CRISPR/Cas12a ultra-specific activation mode based on toehold-mediated strand displacement and branch migration
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Ultrasensitive Point-of-Care Detection of Protein Markers Using an Aptamer-CRISPR/Cas12a-Regulated Liquid Crystal Sensor (ALICS)
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Harnessing Advanced Molecular and Digital Tools for Early Detection of Vector‐Borne Pathogens in Mosquitoes to Control the Emergence and Resurgence of Infectious Diseases
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Journal of Parasitology Research, 2026, 2026
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Molecular reporters for CRISPR/Cas: From design principles to engineering for bioanalytical and diagnostic applications
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CRISPR/Cas9 and Chlorophyll Coordination Micelles for Cancer Treatment by Genome Editing and Photodynamic Therapy
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Inconsistent treatments of the kinetics of Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) impair assessment of its diagnostic potential
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Signal Amplification by the trans-Cleavage Activity of CRISPR-Cas Systems: Kinetics and Performance
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Anal. Chem., 2023, 95, 206
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Spatial double-layer hydrogels enabled visual detection of Cladobotryum mycophilum based on recombinase-aided amplification - CRISPR/Cas12a
Fan’ge Kong, Shichao Peng, Yuchong Zhang, He Zhang, Jiasi Wang and Di Wang
International Journal of Biological Macromolecules, 2025, 308, 142304
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Development of Nucleic‐Acid‐Based Electrochemical Biosensors for Clinical Applications
Zijie Zhang, Payel Sen, Bal Ram Adhikari, Yingfu Li and Leyla Soleymani
Angewandte Chemie, 2022, 134
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Point‐of‐care CRISPR/Cas biosensing technology: A promising tool for preventing the possible COVID‐19 resurgence caused by contaminated cold‐chain food and packaging
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Food Frontiers, 2023, 4, 207
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Advances in the Application of CRISPR/Cas Systems in Molecular Diagnostics
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Synthetic Biology and Engineering, 2026, 4, 10005
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Regulating the trans-Cleavage Activity of CRISPR/Cas12a by Using an Elongation-Caged Single-Stranded DNA Activator and the Biosensing Applications
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Anal. Chem., 2023, 95, 12169
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CRISPR/Cas12a-Responsive Hydrogels for Conjugation-Free and Universal Indicator Release in Colorimetric Detection
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Anal. Chem., 2023, 95, 18522
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CRISPR/Cas12a-based hypochlorous acid and myeloperoxidase biosensors designed on RESET effect
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Sensors and Actuators B: Chemical, 2023, 376, 133000
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Enhanced CRISPR-Cas12a Fluorescent Assay for Detecting Trace Levels of Procalcitonin
Sathishkumar Munusamy, Rana Jahani, Haiyan Zheng, Jun Chen, Juanhua Kong, Yuan Zhao, Lorelei Guan, Shuo Zhou and Xiyun Guan
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CRISPR/Cas System-Based Fluorescent Sensor for Analysis and Detection
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Critical Reviews in Analytical Chemistry, 2025, 1
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Bis-enzyme cascade CRISPR-Cas12a platform for miRNA detection
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Talanta, 2023, 252, 123837
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Engineering Gene-Specific DNAzymes for Accessible and Multiplexed Nucleic Acid Testing
Lu Gao, Ke Yi, Yun Tan, Chen Guo, Danxi Zheng, Chenlan Shen and Feng Li
JACS Au, 2024, 4, 1664
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CRISPR/Cas12a Corona Nanomachine for Detecting Circulating Tumor Nucleic Acids in Serum
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Anal. Chem., 2024, 96, 20074
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Nano-based techniques: A revolutionary approach to prevent covid-19 and enhancing human awareness
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Journal of Drug Delivery Science and Technology, 2023, 86, 104567
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A nanopore-based label-free CRISPR/Cas12a system for portable and ultrasensitive detection of zearalenone
Ziye Pei, Zhuoqun Su, Jianing Chen, Wenrui Li, Di Wu, Lin Li, Yongning Wu and Guoliang Li
Analytica Chimica Acta, 2024, 1330, 343280
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Programming activation of Cas12a via PAM remodeling for on-demand detection of DNA repair enzymes
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Sensors and Actuators B: Chemical, 2026, 454, 139610
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Allosteric Activator-Regulated CRISPR/Cas12a System Enables Biosensing and Imaging of Intracellular Endogenous and Exogenous Targets
Qing-Nan Li, Ai-Xin Ma, Dong-Xia Wang, Zhi-Qi Dai, Shun-Li Wu, Sha Lu, Li−Na Zhu, Hong-Xin Jiang, Dai-Wen Pang and De-Ming Kong
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Enhanced Two-Step LAMP-CRISPR Assay with an Engineered Zst Polymerase for Contamination-Free and Ultrasensitive DNA Detection
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Anal. Chem., 2024
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CRISPR/Cas13a-based colorimetric biosensing platform for point-of-care detection of viral nucleic acids
Zhipeng Zhang, Tianran Zhang, Zimeng Li and Zhijun Zeng
Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy, 2026, 348, 127221
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CRISPR-Cas systems mediated biosensing and applications in food safety detection
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Critical Reviews in Food Science and Nutrition, 2024, 64, 2960
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Recombinase Polymerase Amplification and Target-Triggered CRISPR/Cas12a Assay for Sensitive and Selective Hepatitis B Virus DNA Analysis Based on Lanthanide Tagging and Inductively Coupled Plasma Mass Spectrometric Detection
Chenxi Zhao, Lijie Du, Jing Hu and Xiandeng Hou
Anal. Chem., 2024, 96, 15059
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Identification and diversity analysis of CRISPR-Cas systems in the pathogenic strains of Clostridium botulinum to create eco-friendly phage preparations
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CRISPR/Cas System-Based Biosensors
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Biosensors, 2026, 16, 117
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Integrating CRISPR-Cas12a with Aptamer as a Logic Gate Biosensing Platform for the Detection of CD33 and CD123
Xinyi Yang, Xiaolong Shi, Chenyu Lv, Wenbin Liu, Fengyue Zhang and Bo Liu
ACS Omega, 2025, 10, 13634
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RPA-CRISPR/Cas12a-Based Detection of Haemophilus parasuis
Kunli Zhang, Zeyi Sun, Keda Shi, Dongxia Yang, Zhibiao Bian, Yan Li, Hongchao Gou, Zhiyong Jiang, Nanling Yang, Pinpin Chu, Shaolun Zhai, Zhanyong Wei and Chunling Li
Animals, 2023, 13, 3317
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Artificial mismatch-assisted Cas12a crRNA splicing mechanism enabling interference-free discrimination of homologous microRNAs with single-nucleotide resolution
Xinrui Fei, Chao Lei, Zhaowei Tian, Xinyu Zhang, Wei Ren and Chenghui Liu
Chinese Chemical Letters, 2026, 112450
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A Robust Expression and Purification Method for Production of SpCas9-GFP-MBP Fusion Protein for In Vitro Applications
Andrea Luciana Fleitas, Mario Señorale and Sabina Vidal
MPs, 2022, 5, 44
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CRISPR/Cas-based nanobiosensors: A reinforced approach for specific and sensitive recognition of mycotoxins
Ma Haowei, Shadi Ebrahimi, Sofiene Mansouri, Sherzod Shukhratovich Abdullaev, Hashem O. Alsaab and Zahraa F. Hassan
Food Bioscience, 2023, 56, 103110
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Amplification-Free CRISPR-Cas System Integrated Centrifugal Digital Microfluidic Platform Developed for Multiplexed Respiratory Pathogen Nucleic Acid Analysis
Jing Zhang, Longjie Li, Yidan Zhu, Kun Qian, Qian Xu, Yuling Qin and Li Wu
Anal. Chem., 2025, 97, 25583
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CRISPR-Cas12a-regulated DNA adsorption and metallization on MXenes as enhanced enzyme mimics for sensitive colorimetric detection of hepatitis B virus DNA
Yu Tao, Ke Yi, Haixia Wang, Hae-Won Kim, Kai Li, Xiang Zhu and Mingqiang Li
Journal of Colloid and Interface Science, 2022, 613, 406
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Pocket lab for the rapid detection of monkeypox virus
Jia Wei, Xianying Meng, Juan Li and Bo Pang
Travel Medicine and Infectious Disease, 2022, 50, 102478
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Reflective diagnostics: A self-learning CRISPR–biosensor–AI platform for adaptive food and health safety monitoring
Jacob Tizhe Liberty
Food and Humanity, 2026, 6, 101005
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Photoactivatable Engineering of CRISPR/Cas9‐Inducible DNAzyme Probe for In Situ Imaging of Nuclear Zinc Ions
Ran Liu, Difei Jiang, Yangfang Yun, Zhe Feng, Fenfen Zheng, Yu Xiang, Huanhuan Fan and Jingjing Zhang
Angewandte Chemie, 2024, 136
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Reprogramming the tumor microenvironment by genome editing for precision cancer therapy
Ke Liu, Jia-Jia Cui, Yan Zhan, Qian-Ying Ouyang, Qi-Si Lu, Dong-Hua Yang, Xiang-Ping Li and Ji-Ye Yin
Mol Cancer, 2022, 21
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CRISPR/RNA Aptamer System Activated by an AND Logic Gate for Biomarker-Driven Theranostics
Qiqi Yang, Ming-Jie Dong, Jianglian Xu, Yi Xing, Yue Wang, Jinlong Yang, Xiangdan Meng, Tianzhen Xie, Yingfu Li and Haifeng Dong
J. Am. Chem. Soc., 2025, 147, 169
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Detection of Cancer Marker Flap Endonuclease 1 Using One-Pot Transcription-Powered Clustered Regularly Interspaced Short Palindromic Repeat/Cas12a Signal Expansion
Sheng Ding, Yinghua Wei, Gangyi Chen, Feng Du, Xin Cui, Xin Huang, Yi Yuan, Juan Dong and Zhuo Tang
Anal. Chem., 2022, 94, 13549
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A dual amplification strategy integrating entropy-driven circuit with Cas14a for sensitive detection of miRNA-10b
Xiaojuan Wang, Peiying Lv, Chenxi Zhao, Nana Yin, Teng Fei, Yang Shu and Jianhua Wang
Sensors and Actuators B: Chemical, 2023, 397, 134666
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PAM‐Engineered Toehold Switches as Input‐Responsive Activators of CRISPR‐Cas12a for Sensing Applications
Neda Bagheri, Alejandro Chamorro, Andrea Idili and Alessandro Porchetta
Angewandte Chemie, 2024, 136
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CRISPR/Cas12a Protein Switch Powered Label-Free Electrochemical Biosensor for Sensitive Viral Protease Detection
Tianyi Zhang, Yingying Zhao, Cong Zhu, Xi Zhu, Xiaohua Zhu, Ye Qiu, Zhou Nie and Chunyang Lei
Anal. Chem., 2025, 97, 8039
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Recent advances on CRISPR/Cas system-enabled portable detection devices for on-site agri-food safety assay
Manyan Qiu, Jingwen Zhang, Lidong Pang, Yu Zhang, Qianyu Zhao, Yujun Jiang, Xinyan Yang and Chaoxin Man
Trends in Food Science & Technology, 2022, 129, 364
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Poly(vinylpyrrolidone)-Enhanced CRISPR-Cas System for Robust Nucleic Acid Diagnostics
Pengfei Liu, Jiayu Zeng, Chengchuan Jiang, Jinlian Du, Ling Jiang, Sheng Li, Fanxu Zeng and Erhu Xiong
Anal. Chem., 2024, 96, 15797
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Origami Paper-Based Immunoassay Device with CRISPR/Cas12a Signal Amplification
Hikaru Suzuki, Guodong Tong, Pabitra Nath, Yuki Hiruta and Daniel Citterio
ACS Sens., 2025, 10, 1811
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Integrating CRISPR and isothermal amplification reactions in single-tubes for ultrasensitive detection of nucleic acids: the SARS-CoV-2 RNA example
Wei Feng, Hang Xiao, Aijiao Yuan, Zhaojia Deng, Xiaoyan Wang, Hanyong Peng and X. Chris Le
Sci. Sin.-Chim, 2022, 52, 1685
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Label-Free Resonance Rayleigh Scattering Amplification for Lipopolysaccharide Detection and Logical Circuit by CRISPR/Cas12a-Driven Guanine Nanowire Assisted Non-Cross-Linking Hybridization Chain Reaction
Zhong Feng Gao, Lin Lin Zheng, Lu Ming Dong, Jin Ze Li, Yizhong Shen, Pu Chen and Fan Xia
Anal. Chem., 2022, 94, 6371
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Label-free chemiluminescent CRISPR/Cas12a biosensing strategy for the detection of nucleic acids and non-nucleic acids utilizing DNAzyme Supernova
Cheng Qin, Xinlan Bian, Wei Lv and Bingzhi Li
Sensors and Actuators B: Chemical, 2024, 402, 135143
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Engineering CRISPR/Cas Systems for High‐Specific Nucleic Acid Detection: Innovations, Challenges and Opportunities
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Expanding Cas12a Activity Control with an RNA G‐Quadruplex at the 5′ end of CRISPR RNA
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Emerging Combination of Hydrogel and Electrochemical Biosensors
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Progress in advanced biosensors for authentication of animal-origin food
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Responsive MXene nanovehicles deliver CRISPR/Cas12a for boolean logic-controlled gene editing
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Harnessing the CRISPR‐Cas13d System for Protein Detection by Dual‐Aptamer‐Based Transcription Amplification
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Point‐of‐Care Diagnosis of Respiratory Viruses at Single‐Nucleotide Resolution with an Autocatalytic Rolling Circle Amplification System
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