Directing evolution of novel ligands by mRNA display

Golnaz Kamalinia, Brian J. Grindel, Terry T. Takahashi, Steven W. Millward and Richard W. Roberts

Chem. Soc. Rev., 2021, 50, 9055
DOI: 10.1039/D1CS00160D

Targeting intracellular protein–protein interactions with macrocyclic peptides

Marina Buyanova and Dehua Pei

Trends in Pharmacological Sciences, 2022, 43, 234
DOI: 10.1016/j.tips.2021.11.008

Utilization of macrocyclic peptides to target protein-protein interactions in cancer

Jiawen Yang, Qiaoliang Zhu, Yifan Wu, Xiaojuan Qu, Haixia Liu, Biao Jiang, Di Ge and Xiaoling Song

Front. Oncol., 2022, 12
DOI: 10.3389/fonc.2022.992171

In vitro selection of macrocyclic peptide inhibitors containing cyclic γ2,4-amino acids targeting the SARS-CoV-2 main protease

Takashi Miura, Tika R. Malla, C. David Owen, Anthony Tumber, Lennart Brewitz, Michael A. McDonough, Eidarus Salah, Naohiro Terasaka, Takayuki Katoh, Petra Lukacik, Claire Strain-Damerell, Halina Mikolajek, Martin A. Walsh, Akane Kawamura, Christopher J. Schofield and Hiroaki Suga

Nat. Chem., 2023, 15, 998
DOI: 10.1038/s41557-023-01205-1

State of the art in (semi-)synthesis of Ubiquitin- and Ubiquitin-like tools

Yara Huppelschoten and Gerbrand J. van der Heden van Noort

Seminars in Cell & Developmental Biology, 2022, 132, 74
DOI: 10.1016/j.semcdb.2021.11.025

Expanding the genetic code: In vivo approaches for incorporating non-proteinogenic monomers

Dongheon Lee, Suk Min Yun and Jong-il Choi

Journal of Microbiology, 2025, 63, e2501005
DOI: 10.71150/jm.2501005

Tackling Undruggable Targets with Designer Peptidomimetics and Synthetic Biologics

Colin S. Swenson, Gunasheil Mandava, Deborah M. Thomas and Raymond E. Moellering

Chem. Rev., 2024, 124, 13020
DOI: 10.1021/acs.chemrev.4c00423

Engineering tRNAs for the Ribosomal Translation of Non-proteinogenic Monomers

Maxwell Sigal, Satomi Matsumoto, Adam Beattie, Takayuki Katoh and Hiroaki Suga

Chem. Rev., 2024, 124, 6444
DOI: 10.1021/acs.chemrev.3c00894

Selective macrocyclic peptide modulators of Lys63-linked ubiquitin chains disrupt DNA damage repair

Ganga B. Vamisetti, Abhishek Saha, Yichao J. Huang, Rajeshwer Vanjari, Guy Mann, Julia Gutbrod, Nabieh Ayoub, Hiroaki Suga and Ashraf Brik

Nat Commun, 2022, 13
DOI: 10.1038/s41467-022-33808-6

Gold(I)-Mediated Rapid Cyclization of Propargylated Peptides via Imine Formation

Rajeshwer Vanjari, Deepanjan Panda, Shaswati Mandal, Ganga B. Vamisetti and Ashraf Brik

J. Am. Chem. Soc., 2022, 144, 4966
DOI: 10.1021/jacs.1c12906

Mechanism of selective recognition of Lys48-linked polyubiquitin by macrocyclic peptide inhibitors of proteasomal degradation

Betsegaw Lemma, Di Zhang, Ganga B. Vamisetti, Bryan G. Wentz, Hiroaki Suga, Ashraf Brik, Jacek Lubkowski and David Fushman

Nat Commun, 2023, 14
DOI: 10.1038/s41467-023-43025-4

Recent Advances in Non‐Standard Macrocyclic Peptide Ligand Discovery using mRNA Display

Yizhen Yin and Christopher John Hipolito

Israel Journal of Chemistry, 2024
DOI: 10.1002/ijch.202300167

Non‐Canonical Amino Acids for Engineering Peptides and Proteins with new Functions

Kelly Zhi Qi Zhou and Richard Obexer

Israel Journal of Chemistry, 2024
DOI: 10.1002/ijch.202400006

Exocyclic and Linker Editing of Lys63‐linked Ubiquitin Chains Modulators Specifically Inhibits Non‐homologous End‐joining Repair

Abhishek Saha, Laila A. Bishara, Yakop Saed, Ganga B. Vamisetti, Shaswati Mandal, Hiroaki Suga, Nabieh Ayoub and Ashraf Brik

Angew Chem Int Ed, 2024, 63
DOI: 10.1002/anie.202409012

The RaPID Platform for the Discovery of Pseudo-Natural Macrocyclic Peptides

Yuki Goto and Hiroaki Suga

Acc. Chem. Res., 2021, 54, 3604
DOI: 10.1021/acs.accounts.1c00391

Direct Selection of Functional De Novo Macrocycles for Activation of On‐Cellulo Insulin Receptor

Yun‐Hsuan Kuo, Emiko Mihara, Junichi Takagi and Hiroaki Suga

Angewandte Chemie, 2026
DOI: 10.1002/ange.202526008

Combining Chemical Protein Synthesis and Random Nonstandard Peptides Integrated Discovery for Modulating Biological Processes

Abhishek Saha, Hiroaki Suga and Ashraf Brik

Acc. Chem. Res., 2023, 56, 1953
DOI: 10.1021/acs.accounts.3c00178

Synthesis and Evaluation of Ubiquitin–Dioxetane Conjugate as a Chemiluminescent Probe for Monitoring Deubiquitinase Activity

Sara Gutkin, Satish Gandhesiri, Ashraf Brik and Doron Shabat

Bioconjugate Chem., 2021, 32, 2141
DOI: 10.1021/acs.bioconjchem.1c00413

The Molecular Toolbox for Linkage Type‐Specific Analysis of Ubiquitin Signaling

Julian Koch, Camilla Reiter Elbæk, Dominik Priesmann and Rune Busk Damgaard

ChemBioChem, 2025, 26
DOI: 10.1002/cbic.202500114

Highly Efficient Cyclization Approach of Propargylated Peptides via Gold(I)-Mediated Sequential C–N, C–O, and C–C Bond Formation

Rajeshwer Vanjari, Emad Eid, Ganga B. Vamisetti, Shaswati Mandal and Ashraf Brik

ACS Cent. Sci., 2021, 7, 2021
DOI: 10.1021/acscentsci.1c00969

Direct Selection of Functional De Novo Macrocycles for Activation of On‐Cellulo Insulin Receptor

Yun‐Hsuan Kuo, Emiko Mihara, Junichi Takagi and Hiroaki Suga

Angew Chem Int Ed, 2026
DOI: 10.1002/anie.202526008

Identification of photocrosslinking peptide ligands by mRNA display

Yuteng Wu, M. Teresa Bertran, Dhira Joshi, Sarah L. Maslen, Catherine Hurd and Louise J. Walport

Commun Chem, 2023, 6
DOI: 10.1038/s42004-023-00898-2

Direct Cellular Screening of Pd-Mediated Arylation of Cyclic Peptide Binders Targeting Ubiquitin Chains: Toward Modulating NEMO Liquid–Liquid Phase Separation

Mahdi Hasan, Nagaraju Vodnala, Yuri Glagovsky, Yakop Saed, Julia Kriegesmann, Hiroaki Suga and Ashraf Brik

J. Am. Chem. Soc., 2025, 147, 28303
DOI: 10.1021/jacs.5c09059

Exocyclic and Linker Editing of Lys63‐linked Ubiquitin Chains Modulators Specifically Inhibits Non‐homologous End‐joining Repair

Abhishek Saha, Laila A. Bishara, Yakop Saed, Ganga B. Vamisetti, Shaswati Mandal, Hiroaki Suga, Nabieh Ayoub and Ashraf Brik

Angewandte Chemie, 2024, 136
DOI: 10.1002/ange.202409012