Themed collection 10th Anniversary of Molecular BioSystems

Richard Kelly introduces the 10th anniversary issue of Molecular BioSystems.

Dozens of intracellular proteins have a second function on the cell surface, referred to as “intracellular/surface moonlighting proteins”. An analysis of the results of 22 cell surface proteomics studies was performed to address whether the hundreds of intracellular proteins found on the cell surface could be candidates for being additional intracellular/surface moonlighting proteins.

Gene expression profiling is a fast, cheap and standardised analysis that provides a high dimensional measurement of the state of a biological sample, including of single cells. Computational methods to reconstruct the composition of samples and spatial and temporal information from expression profiles are described, as well as how they can be used to describe the effects of genetic variation.

Technological advances have enabled multi-dimensional analyses of gene expression regulation and its dynamics.

A STRING analysis of proteins containing clusters of 3 or more adjacent phosphoserine residues reveals that the great majority of these proteins are interconnected by physical and/or functional links to generate a kind of “phosphoclusterome”.

With the expanding interest in RNA biology, interest in artificial RNA-binding proteins (RBPs) is likewise increasing.

Delivering advanced therapies for brain cancer based on RNAi.

With the avalanche of DNA/RNA sequences generated in the post-genomic age, it is urgent to develop automated methods for analyzing the relationship between the sequences and their functions.

We review the progress in the prediction of protein methylation sites in the past 10 years and discuss the challenges that are faced while developing novel predictors in the future.

Enzyme inhibitory peptides with a loop structure stabilized using an antiparallel β-sheet scaffold (β-loop peptide) were obtained from a designed peptide phage library.

A photoaffinity library expedited the discovery of a site-specific DcpS probe.

While using a serine (S) as linker for circularization increases the thermostability, a longer linker (RGKCWE) leads to reduced aggregation after heat shock at elevated temperatures.

Euglena gracilis is a highly complex green alga. In evolutionary terms is most closely related to the protozoan parasites Trypanosoma and Leishmania but it also shows characteristics of both plants and animals.

Human genes with a greater number of transcript variants are more likely to play functionally important roles such as cellular maintenance and survival.

Our work reveals that simple reversible protein–protein interactions, when embedded into signalling cascades, give rise to extremely rich and complex regulatory dynamics in the absence of explicit positive and negative feedbacks.

The local dynamics of host and viral peptide motifs suggests a different scenario for partner recognition. Host peptide motifs serve as molecular recognition elements, while viral motifs preserve the structural heterogeneity and remain fuzzy when bound to the host.

We characterized a new resin for the enrichment of multiple phosphodiesterases and their interactors.

A range of experimental approaches are used to understand the HIF-1α/p300 interface – an important anticancer drug development target – in greater detail.

HIF-1-mediated hypoxia signalling in embryonic tissue is probed by assessing the methylation status of the HIF-1α and EPO HRE promoters using bisulfite sequencing.

A large library of macrocyclic peptides containing multiple N-alkylated structures was prepared as one-bead one-compound format.

Platensimycin and platencin congeners from overproducing strains support PtmO4 as a long-chain acyl-CoA dehydrogenase catalyzing β-oxidation of the diterpenoid intermediates.

Imidazole-based synthetic small molecules promote neurogenesis in readily available and simply manageable neuroblastoma and fibroblast cells.

Az blocks the association of Hsc70 with clathrin and consequently suppresses the disassembly of CCVs during clathrin-mediated endocytosis.

iSBatch: an ImageJ plugin for fast evaluation of analysis pipelines and visual exploration of datasets.