Themed collection Lab on a Chip Recent HOT Articles
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Enabling high-throughput single-animal gene-expression studies with molecular and micro-scale technologies
How can microfluidics address the significant limitations to the current tools that measure gene expression in single-animal studies?
Lab Chip, 2020,20, 4528-4538
https://doi.org/10.1039/D0LC00881H
![Open Access](https://www.rsc-cdn.org/pubs-core/2022.0.147/content/NewImages/open-access-icon-orange.png)
Interfacing cells with organic transistors: a review of in vitro and in vivo applications
This review focuses on the applications of organic transistors in cellular interfacing. It offers a comprehensive retrospective of the past, an overview of the latest innovations, and a glance on the future perspectives of this fast-evolving field.
Lab Chip, 2021,21, 795-820
https://doi.org/10.1039/D0LC01007C
Tumor-on-a-chip platforms to study cancer–immune system crosstalk in the era of immunotherapy
Immunotherapy is a powerful therapeutic approach able to re-educate the immune system to fight cancer. In this scenario, tumor-on-chip can help to choose the right immunotherapeutic treatment.
Lab Chip, 2021,21, 234-253
https://doi.org/10.1039/D0LC00799D
Single-cell microfluidic impedance cytometry: from raw signals to cell phenotypes using data analytics
Review of chip designs and data analytics to stratify heterogeneity in cellular systems via microfluidic impedance cytometry.
Lab Chip, 2021,21, 22-54
https://doi.org/10.1039/D0LC00840K
Trapping and control of bubbles in various microfluidic applications
Active and passive techniques for bubble trapping and control in various microfluidic applications.
Lab Chip, 2020,20, 4512-4527
https://doi.org/10.1039/D0LC00906G
Phase separation of a nonionic surfactant aqueous solution in a standing surface acoustic wave for submicron particle manipulation
Acoustic induced nanoparticle patterning and location migration in inhomogeneous media formed in situ.
Lab Chip, 2021,21, 660-667
https://doi.org/10.1039/D0LC00986E
A facile multi-material direct laser writing strategy
We present an accessible strategy for printing multi-material 3D nanostructured components via microfluidic two-photon direct laser writing.
Lab Chip, 2019,19, 2340-2345
https://doi.org/10.1039/C9LC00398C
A miniaturized optical tomography platform for volumetric imaging of engineered living systems
Volumetric optical microscopy approaches that enable acquisition of three-dimensional (3D) information from a biological sample are attractive for numerous non-invasive imaging applications.
Lab Chip, 2019,19, 550-561
https://doi.org/10.1039/C8LC01190G
Microfluidics-enabled rational design of immunomagnetic nanomaterials and their shape effect on liquid biopsy
A microfluidics-enabled strategy for the controllable synthesis of immunomagnetic nanomaterials was developed, and the shape-dependent screening efficiency of CTCs was investigated.
Lab Chip, 2018,18, 1997-2002
https://doi.org/10.1039/C8LC00273H
Dynamic pneumatic rails enabled microdroplet manipulation
This study presented a convenient method of gathering, splitting, merging, and sorting microdroplets by dynamic pneumatic rails in double-layered microfluidic devices.
Lab Chip, 2021,21, 105-112
https://doi.org/10.1039/D0LC00805B
A high-throughput multiplexed microfluidic device for COVID-19 serology assays
A microfluidic device to quantitate antibody reactivities to SARS-CoV-2 antigens: spike protein, RDB, S1 subunit, and nucleocapsid, from 50 serum samples.
Lab Chip, 2021,21, 93-104
https://doi.org/10.1039/D0LC01068E
An integrated microfluidic system for early detection of sepsis-inducing bacteria
Isolation and identification of bacteria from human blood were automated on an integrated microfluidic system for sepsis-inducing bacteria.
Lab Chip, 2021,21, 113-121
https://doi.org/10.1039/D0LC00966K
A high-throughput cell culture system based on capillary and centrifugal actions for rapid antimicrobial susceptibility testing
A capillary and centrifuge-based rapid antimicrobial susceptibility testing system is developed to reduce the time of loading the sample and culture media while achieving a high-throughput testing capacity.
Lab Chip, 2020,20, 4552-4560
https://doi.org/10.1039/D0LC00753F
![Open Access](https://www.rsc-cdn.org/pubs-core/2022.0.147/content/NewImages/open-access-icon-orange.png)
Modular off-chip emulsion generator enabled by a revolving needle
An integrated revolving needle emulsion generator (RNEG) is developed to achieve high-throughput production of monodispersed droplets in an off-chip manner.
Lab Chip, 2020,20, 4592-4599
https://doi.org/10.1039/D0LC00939C
Everything in its right place: controlling the local composition of hydrogels using microfluidic traps
We introduce a microfluidic Hele-Shaw trapping cell that enables the fabrication of hydrogel sheets whose composition can be abruptly and controllably changed.
Lab Chip, 2020,20, 4572-4581
https://doi.org/10.1039/D0LC00691B
Soft elastomeric composite materials with skin-inspired mechanical properties for stretchable electronic circuits
An elastomeric composite material with skin-like mechanical responses and spatially heterogeneous rigidity is developed to accommodate stretchable electronic circuit systems.
Lab Chip, 2019,19, 2709-2717
https://doi.org/10.1039/C9LC00544G
Intelligent whole-blood imaging flow cytometry for simple, rapid, and cost-effective drug-susceptibility testing of leukemia
The drug susceptibility of leukemia cells in whole blood is evaluated by using extreme-throughput imaging flow cytometry with deep learning.
Lab Chip, 2019,19, 2688-2698
https://doi.org/10.1039/C8LC01370E
![Open Access](https://www.rsc-cdn.org/pubs-core/2022.0.147/content/NewImages/open-access-icon-orange.png)
Clinical translation of microfluidic sensor devices: focus on calibration and analytical robustness
Modular platform providing the building blocks to translate specialised microfluidics from the laboratory to a clinical environment.
Lab Chip, 2019,19, 2537-2548
https://doi.org/10.1039/C9LC00400A
The biomolecular corona of gold nanoparticles in a controlled microfluidic environment
The use of microfluidic devices may allow standardizing corona formation protocols and comparing data from different laboratories.
Lab Chip, 2019,19, 2557-2567
https://doi.org/10.1039/C9LC00341J
![Open Access](https://www.rsc-cdn.org/pubs-core/2022.0.147/content/NewImages/open-access-icon-orange.png)
A nanochannel through a plasmonic antenna gap: an integrated device for single particle counting
A gold bowtie nanoantenna integrated with a 30 nm × 30 nm nanochannel for single particle detection and counting in real time.
Lab Chip, 2019,19, 2394-2403
https://doi.org/10.1039/C9LC00186G
Measurement of the magnetic susceptibility of subtle paramagnetic solutions using the diamagnetic repulsion of polymer microparticles
The diamagnetic repulsion of polymer microspheres in a microfluidic platform enables the discrimination of magnetic susceptibility of subtle paramagnetic solutions.
Lab Chip, 2019,19, 2356-2361
https://doi.org/10.1039/C9LC00245F
Remotely controlled nanofluidic implantable platform for tunable drug delivery
An implantable nanofluidic device for remote controlled drug delivery.
Lab Chip, 2019,19, 2192-2204
https://doi.org/10.1039/C9LC00394K
Two-dimensional planar swimming selects for high DNA integrity sperm
Selection of high DNA integrity sperm via planar swimming in a microfluidic device outperforms clinically established sperm preparation.
Lab Chip, 2019,19, 2161-2167
https://doi.org/10.1039/C9LC00209J
Controlled co-precipitation of biocompatible colorant-loaded nanoparticles by microfluidics for natural color drinks
Controlled co-precipitation of biocompatible colorant-loaded nanoparticles in microfluidic channels for natural color drinks.
Lab Chip, 2019,19, 2089-2095
https://doi.org/10.1039/C9LC00240E
Microfluidic blood vasculature replicas using backside lithography
A one-mask microfabrication process to build artificial vascular networks for the analysis of blood flows and hemophysics.
Lab Chip, 2019,19, 2096-2106
https://doi.org/10.1039/C9LC00254E
![Open Access](https://www.rsc-cdn.org/pubs-core/2022.0.147/content/NewImages/open-access-icon-orange.png)
Rapid separation and identification of beer spoilage bacteria by inertial microfluidics and MALDI-TOF mass spectrometry
Microfluidics and MALDI-TOF MS is a rapid, high-throughput, and accurate method for the identification of beer spoilage bacteria.
Lab Chip, 2019,19, 1961-1970
https://doi.org/10.1039/C9LC00152B
Condensing-enriched magnetic photonic barcodes on superhydrophobic surface for ultrasensitive multiple detection
When PhC barcodes with stable characteristic reflection peaks are integrated with a superhydrophobic substrate they can realize the ultrasensitive multiple detection by the enrichment of the solution upon evaporation. Thus, the detection of miRNA was carried out by the system and it was found the detection limit was improved by about three orders.
Lab Chip, 2019,19, 1783-1789
https://doi.org/10.1039/C9LC00223E
Gel-on-a-chip: continuous, velocity-dependent DNA separation using nanoscale lateral displacement
We studied the dsDNA trajectories being advected while diffusing in a pressure driven flow in a nanoDLD array.
Lab Chip, 2019,19, 1567-1578
https://doi.org/10.1039/C8LC01408F
Traction microscopy with integrated microfluidics: responses of the multi-cellular island to gradients of HGF
Microfluidic system integrated with cell collectives and traction microscopy demonstrates that collective cell migration plays a central role in development, regeneration, and metastasis.
Lab Chip, 2019,19, 1579-1588
https://doi.org/10.1039/C9LC00173E
![Open Access](https://www.rsc-cdn.org/pubs-core/2022.0.147/content/NewImages/open-access-icon-orange.png)
High-throughput single-particle detections using a dual-height-channel-integrated pore
We report a proof-of-principle demonstration of particle concentration to achieve high-throughput resistive pulse detections of bacteria using a microfluidic-channel-integrated micropore.
Lab Chip, 2019,19, 1352-1358
https://doi.org/10.1039/C8LC01371C
Sorting by interfacial tension (SIFT): label-free selection of live cells based on single-cell metabolism
A new label-free and passive microfluidic technique to select cells based on single-cell glycolytic activity. The method has broad applicability and we demonstrate here single-cell droplet encapsulation and selection of live cells.
Lab Chip, 2019,19, 1344-1351
https://doi.org/10.1039/C8LC01328D
Tailoring surface acoustic wave atomisation for cryo-electron microscopy sample preparation
Surface acoustic wave (SAW) atomisation is investigated in the context of cryo electron microscopy grid preparation. Here, the primary requirements are a reproducible and narrow plume of droplets delivering a low fluid flow rate.
Lab Chip, 2019,19, 1378-1385
https://doi.org/10.1039/C8LC01347K
High-throughput mechanotransduction in Drosophila embryos with mesofluidics
A mesofluidic device allows alignment, immobilization, compression, imaging, and recovery of hundreds of live Drosophila embryos to investigate mechanotransduction.
Lab Chip, 2019,19, 1141-1152
https://doi.org/10.1039/C8LC01055B
On-chip stool liquefaction via acoustofluidics
An acoustofluidic chip can liquefy stool samples in a continuous flow.
Lab Chip, 2019,19, 941-947
https://doi.org/10.1039/C8LC01310A
![Open Access](https://www.rsc-cdn.org/pubs-core/2022.0.147/content/NewImages/open-access-icon-orange.png)
Modular soft robotic microdevices for dexterous biomanipulation
We present a methodology for building biologically inspired, soft microelectromechanical systems (MEMS) devices.
Lab Chip, 2019,19, 778-788
https://doi.org/10.1039/C8LC01200H
![Open Access](https://www.rsc-cdn.org/pubs-core/2022.0.147/content/NewImages/open-access-icon-orange.png)
Latchable microfluidic valve arrays based on shape memory polymer actuators
Latchable microfluidic valve arrays taking advantage of the shape memory polymer's multiple stable shapes and large change in stiffness for a small temperature variation.
Lab Chip, 2019,19, 608-617
https://doi.org/10.1039/C8LC01024B
Plastic-based acoustofluidic devices for high-throughput, biocompatible platelet separation
A plastic-based acoustofluidic device for high-throughput platelet separation consisting of a transducer and a disposable chip.
Lab Chip, 2019,19, 394-402
https://doi.org/10.1039/C8LC00527C
Pumpless platform for high-throughput dynamic multicellular culture and chemosensitivity evaluation
We report here a 96 well-plate based pumpless platform for high-throughput dynamic multicellular culture and chemosensitivity evaluation.
Lab Chip, 2019,19, 254-261
https://doi.org/10.1039/C8LC00872H
Natural gas vaporization in a nanoscale throat connected model of shale: multi-scale, multi-component and multi-phase
Production of hydrocarbons from shale is a complex process that necessitates the extraction of multi-component hydrocarbons trapped in multi-scale nanopores.
Lab Chip, 2019,19, 272-280
https://doi.org/10.1039/C8LC01053F
Versatile platform for performing protocols on a chip utilizing surface acoustic wave (SAW) driven mixing
We present a dextrous microfluidic device which features a reaction chamber with volume flexibility and acoustic mixing.
Lab Chip, 2019,19, 262-271
https://doi.org/10.1039/C8LC01117F
A microfluidic co-cultivation platform to investigate microbial interactions at defined microenvironments
A microfluidic device for the co-cultivation of microbial cells.
Lab Chip, 2019,19, 98-110
https://doi.org/10.1039/C8LC00977E
Urine-based liquid biopsy: non-invasive and sensitive AR-V7 detection in urinary EVs from patients with prostate cancer
Detection of AR-V7 in urinary EVs provides a simple and promising liquid biopsy tool for patients with prostate cancer.
Lab Chip, 2019,19, 87-97
https://doi.org/10.1039/C8LC01185K
Multiplexed immunoassay using post-synthesis functionalized hydrogel microparticles
Post-synthesis functionalized hydrogel microparticles were demonstrated in multiplex immunoassays with high sensitivity, a wide assay range, and fast detection.
Lab Chip, 2019,19, 111-119
https://doi.org/10.1039/C8LC01160E
High-throughput, non-equilibrium studies of single biomolecules using glass-made nanofluidic devices
Single-molecule detection schemes offer powerful means to overcome static and dynamic heterogeneity inherent to complex samples.
Lab Chip, 2019,19, 79-86
https://doi.org/10.1039/C8LC01175C
![Open Access](https://www.rsc-cdn.org/pubs-core/2022.0.147/content/NewImages/open-access-icon-orange.png)
Microfluidic bead encapsulation above 20 kHz with triggered drop formation
Bead-triggered breakup of a fluid jet into monodispersed droplets improves the throughput of bead-based droplet workflows, enabling the analysis of large populations and the detection of rare events.
Lab Chip, 2018,18, 3598-3605
https://doi.org/10.1039/C8LC00514A
Extraction of electrokinetically separated analytes with on-demand encapsulation
On-demand two-phase encapsulation of electrokinetically separated analytes decouples electrokinetic separations from downstream analytical processes.
Lab Chip, 2018,18, 3588-3597
https://doi.org/10.1039/C8LC00912K
Selective assembly and functionalization of miniaturized redox capacitor inside microdevices for microbial toxin and mammalian cell cytotoxicity analyses
We report a novel strategy for bridging information transfer between electronics and biological systems within microdevices.
Lab Chip, 2018,18, 3578-3587
https://doi.org/10.1039/C8LC00583D
Determining the lipid specificity of insoluble protein transmembrane domains
Overcoming solubility of membrane protein transmembrane domains to study their specificity to lipid compositions.
Lab Chip, 2018,18, 3561-3569
https://doi.org/10.1039/C8LC00311D
Novel devices for studying acute and chronic mechanical stress in retinal pigment epithelial cells
Replicating mechanical stress using two novel devices revealed that mechanical stress in retinal pigment epithelial cells induces choroidal neovascularization.
Lab Chip, 2018,18, 3413-3424
https://doi.org/10.1039/C8LC00659H
Measurement and mitigation of free convection in microfluidic gradient generators
Microfluidic gradient generators are used to study the movement of living cells, lipid vesicles, and colloidal particles in response to spatial variations in their local chemical environment.
Lab Chip, 2018,18, 3371-3378
https://doi.org/10.1039/C8LC00526E
Integrated measurement of intracellular proteins and transcripts in single cells
Single cell coupled proteomic and transcriptomic measurements are captured on a scalable microfluidic device.
Lab Chip, 2018,18, 3251-3262
https://doi.org/10.1039/C8LC00639C
![Open Access](https://www.rsc-cdn.org/pubs-core/2022.0.147/content/NewImages/open-access-icon-orange.png)
3D microfluidic ex vivo culture of organotypic tumor spheroids to model immune checkpoint blockade
Microfluidic culture has the potential to revolutionize cancer diagnosis and therapy.
Lab Chip, 2018,18, 3129-3143
https://doi.org/10.1039/C8LC00322J
A microfluidic approach to study the effect of mechanical stress on erythrocytes in sickle cell disease
This work presents a microfluidic device to challenge the deformability of normal and pathological red cells and their resistance to lysis upon mechanical stress.
Lab Chip, 2018,18, 2975-2984
https://doi.org/10.1039/C8LC00637G
Localized mechanical stimulation of single cells with engineered spatio-temporal profile
We introduce a new platform for mechanobiology based on active substrates, made of Fe-coated polymeric micropillars, capable to apply mechanical stimuli with tunable spatio-temporal profile on a cell culture.
Lab Chip, 2018,18, 2955-2965
https://doi.org/10.1039/C8LC00393A
Automated microfluidic droplet sampling with integrated, mix-and-read immunoassays to resolve endocrine tissue secretion dynamics
A fully automated droplet generation and analysis device based on pressure driven push-up valves for precise pumping of fluid and volumetric metering has been developed for high resolution hormone secretion sampling and measurement.
Lab Chip, 2018,18, 2926-2935
https://doi.org/10.1039/C8LC00616D
Detecting miRNA biomarkers from extracellular vesicles for cardiovascular disease with a microfluidic system
A novel microfluidic platform for extracellular vesicle extraction, microRNA isolation and detection with field-effect transistors for early detection of cardiovascular diseases.
Lab Chip, 2018,18, 2917-2925
https://doi.org/10.1039/C8LC00386F
Micro-droplet arrays for micro-compartmentalization using an air/water interface
Here we present a water-in-air droplet platform for micro-compartmentalization for single molecule guided synthesis and analysis consisting of a flow-system hosting dense arrays of aqueous microdroplets on a glass surface surrounded by air.
Lab Chip, 2018,18, 2797-2805
https://doi.org/10.1039/C8LC00608C
A system to monitor statin-induced myopathy in individual engineered skeletal muscle myobundles
Microphysiological tissue engineering models of human skeletal muscle (myobundles) in series with a calibrated elastic membrane provide a platform to investigate the mechanism of muscle diseases and to study the response to drugs and toxins in vitro.
Lab Chip, 2018,18, 2787-2796
https://doi.org/10.1039/C8LC00654G
Microfluidic filter device with nylon mesh membranes efficiently dissociates cell aggregates and digested tissue into single cells
Tissues are increasingly being analyzed at this single cell level. We present a simple and inexpensive microfluidic filter device that can rapidly and effectively improve the quality of single cell suspensions obtained from digested tissue samples.
Lab Chip, 2018,18, 2776-2786
https://doi.org/10.1039/C8LC00507A
UniChip enables long-term recirculating unidirectional perfusion with gravity-driven flow for microphysiological systems
UniChip enables recirculating unidirectional perfusion with gravity-driven flow, facilitating reliable and cost-effective integration of shear stress-sensitive tissues into microphysiological systems.
Lab Chip, 2018,18, 2563-2574
https://doi.org/10.1039/C8LC00394G
Engineering cell heterogeneity into organs-on-a-chip
To improve predictive efficacy of organ-on-a-chip devices, developers must consider cell heterogeneity.
Lab Chip, 2018,18, 2378-2395
https://doi.org/10.1039/C8LC00413G
![Open Access](https://www.rsc-cdn.org/pubs-core/2022.0.147/content/NewImages/open-access-icon-orange.png)
Quantitative and multiplex microRNA assays from unprocessed cells in isolated nanoliter well arrays
This platform utilizes functionalized hydrogel posts contained within isolated nanoliter well reactors for quantitative and multiplex microRNA assays directly from unprocessed cell samples without needing prior nucleic acid extraction.
Lab Chip, 2018,18, 2410-2424
https://doi.org/10.1039/C8LC00498F
NemaFlex: a microfluidics-based technology for standardized measurement of muscular strength of C. elegans
NemaFlex measures a behavior- and gait-independent measure of C. elegans strength analogous to maximum voluntary force in humans.
Lab Chip, 2018,18, 2187-2201
https://doi.org/10.1039/C8LC00103K
Microstructure guided multi-scale liquid patterning on an open surface
A simple and fast multi-scale microliquid patterning on an open surface is demonstrated.
Lab Chip, 2018,18, 2013-2022
https://doi.org/10.1039/C7LC01288H
A multiplexed microfluidic system for evaluation of dynamics of immune–tumor interactions
A system for perfusing and interacting tumor fragments and immune cells and testing drug response with image analytics is reported.
Lab Chip, 2018,18, 1844-1858
https://doi.org/10.1039/C8LC00256H
Isolation and genome sequencing of individual circulating tumor cells using hydrogel encapsulation and laser capture microdissection
Single CTC sequencing workflow using biophysical enrichment and single cell isolation by laser capture microdissection.
Lab Chip, 2018,18, 1736-1749
https://doi.org/10.1039/C8LC00184G
Double-sided 3D printing on paper towards mass production of three-dimensional paper-based microfluidic analytical devices (3D-μPADs)
Recently, much effort has been focused on developing three-dimensional, paper-based microfluidic analytical devices (3D-μPADs) targeting in vitro diagnostics.
Lab Chip, 2018,18, 1533-1538
https://doi.org/10.1039/C8LC00367J
![Open Access](https://www.rsc-cdn.org/pubs-core/2022.0.147/content/NewImages/open-access-icon-orange.png)
Multiparameter cell-tracking intrinsic cytometry for single-cell characterization
We introduce a multiparameter intrinsic cytometry approach for single-cell characterization that combines ≥2 label-free measurement techniques onto the same platform.
Lab Chip, 2018,18, 1430-1439
https://doi.org/10.1039/C8LC00240A
Injection molded lab-on-a-disc platform for screening of genetically modified E. coli using liquid–liquid extraction and surface enhanced Raman scattering
We present an injection molded lab-on-a-disc enabling quantification of bacterial metabolites in E. coli supernatant.
Lab Chip, 2018,18, 869-877
https://doi.org/10.1039/C7LC01217A
Engineering reaction–diffusion networks with properties of neural tissue
The application of soft lithography to create reaction–diffusion networks capable of generating spatiotemporal patterns analogous to biological central pattern generators.
Lab Chip, 2018,18, 714-722
https://doi.org/10.1039/C7LC01187C
About this collection
Here is a collection of recent Lab on a Chip articles which have been classified as ‘HOT articles’ due to receiving particularly high scores at peer review.