Issue 37, 2022

One step cascade detection of galactose based on a galactose oxidase-composited peroxidase nanozyme

Abstract

Abnormal galactose metabolism is the main cause of galactosemia, which makes the accurate and rapid analysis of galactose levels in food and organism the key issue at present. In this study, a novel strategy for one-step galactose determination was proposed based on galactose oxidase and copper-based metal–organic framework complexes (GAOx@MOF) with dual catalytic activities at neutral pH. Typically, GAOx catalyzes the oxidation of the C6 hydroxyl group of D-galactose to generate an aldehyde (D-galactose-hexanedial), and coupled with the reduction of dioxygen to H2O2, which was immediately transformed to ˙OH by mimicking peroxidase activity and at the same time oxidized ABTS to a green product with a clear colorimetric signal. The whole process was completed using one buffer, which simplified the procedure and increased the sensitivity. Moreover, the proposed method can also be used for the quantitative analysis of galactose. It showed a good linear relationship at 20–1000 μM, while the LOD was 6.67 μM. Furthermore, the strategy has been successfully utilized for galactose determination in milk samples, which proved its promising applications in clinical analysis and the food industry.

Graphical abstract: One step cascade detection of galactose based on a galactose oxidase-composited peroxidase nanozyme

Article information

Article type
Paper
Submitted
01 Eost 2022
Accepted
30 Eost 2022
First published
30 Eost 2022

Anal. Methods, 2022,14, 3644-3651

One step cascade detection of galactose based on a galactose oxidase-composited peroxidase nanozyme

H. Huang, D. Song, W. Zhang, Y. Sun and Y. Li, Anal. Methods, 2022, 14, 3644 DOI: 10.1039/D2AY01224C

To request permission to reproduce material from this article, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.

Social activity

Spotlight

Advertisements