Issue 13, 2022

Recent progress in electrochemiluminescence microscopy analysis of single cells

Abstract

Electrochemiluminescence (ECL) is produced by chemiluminescence reactions initiated by electrochemical methods. On this basis, ECL microscopy (ECLM) is an emerging visualization tool to directly study local electrochemical features with good spatial and temporal resolution. Recent progress in ECLM demonstrates that it is particularly important in single-cell studies on the morphological structures and functional characteristics of cells due to the unique heterogeneity of cells. Compared with other single-cell imaging methods, ECLM possesses advantages such as a low optical background, high sensitivity, simple instrumentation and surface-confined mode. In this mini-review, we discuss the recent development of single-cell ECLM and classify these works according to different ECL routes. Because ECL routes significantly influence the features of ECLM, such as the thickness of the emitting layer and the brightness of emitters, it is necessary to provide a new insight to revisit these works and discuss what ECL routes can be chosen to study single-cell processes from different perspectives. Considering the gradual growth in single-cell ECLM, we show the developmental trajectory and how these works connect with one another. In addition, the coexisting challenges as well as opportunities inspire us to design more ECLM methods to investigate local electrochemical phenomena.

Graphical abstract: Recent progress in electrochemiluminescence microscopy analysis of single cells

Article information

Article type
Minireview
Submitted
26 Ebr. 2022
Accepted
28 Mae 2022
First published
30 Mae 2022

Analyst, 2022,147, 2884-2894

Recent progress in electrochemiluminescence microscopy analysis of single cells

Z. Zhang, C. Ma, Q. Xu and J. Zhu, Analyst, 2022, 147, 2884 DOI: 10.1039/D2AN00709F

To request permission to reproduce material from this article, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.

Social activity

Spotlight

Advertisements