Secondary orbital interaction in action: DFT calculations in combination with the distortion–interaction model help to uncover underlying factors responsible for the increased reactivity of sulfone- and sulfoxide-substituted tetrazines in iEDDA.
We formalized a unique working mechanism – internal conversion to a dark state (ICDS) to rationalize the fluorogenicity of the tetrazine-fused fluorophores with integrated π-conjugations.
Tetrazine-based linkers as intrinsically tagged alternatives to integrate post-synthetic framework modification with click chemistry reactivity.
This review highlights the mechanisms underlying the unique fluorogenic properties of tetrazine-functionalized labels and proposes new strategies to enhance fluorogenicity in the near-infrared region.
Microfluidic on-chip production of polymeric hydrogel microspheres (MPs) can be designed for the loading of different biologically active cargos and living cells.