Issue 24, 2018

A high-throughput serum Raman spectroscopy platform and methodology for colorectal cancer diagnostics

Abstract

Vibrational spectroscopic techniques such as Raman spectroscopy and Fourier transform infrared spectroscopy (FTIR) have huge potential for the analysis of biological specimens. The techniques allow the user to gain label-free, non-destructive biochemical information about a given sample. Previous studies using vibrational spectroscopy with the specific application of diagnosing colorectal diseases such as cancer have mainly focused on in vivo or in vitro studies of tissue specimens using microscopy or probe based techniques. There have been few studies of vibrational spectroscopic techniques based on the analysis of blood serum for the advancement of colorectal cancer diagnostics. With growing interest in the field of liquid biopsies, this study presents the development of a high-throughput (HT) serum Raman spectroscopy platform and methodology and compares dry and liquid data acquisition of serum samples. This work considers factors contributing to translatability of the methodologies such as HT design, inter-user variability and sample handling effects on diagnostic capability. The HT Raman methods were tested on a pilot dataset of serum from 30 cancer patients and 30 matched control patients using statistical analysis via cross-validated PLS-DA with a maximum achieved a sensitivity of 83% and specificity of 83% for detecting colorectal cancer.

Graphical abstract: A high-throughput serum Raman spectroscopy platform and methodology for colorectal cancer diagnostics

Article information

Article type
Paper
Submitted
16 জুলাই 2018
Accepted
23 অক্টো. 2018
First published
01 নভে. 2018

Analyst, 2018,143, 6014-6024

A high-throughput serum Raman spectroscopy platform and methodology for colorectal cancer diagnostics

C. A. Jenkins, R. A. Jenkins, M. M. Pryse, K. A. Welsby, M. Jitsumura, C. A. Thornton, P. R. Dunstan and D. A. Harris, Analyst, 2018, 143, 6014 DOI: 10.1039/C8AN01323C

To request permission to reproduce material from this article, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.

Social activity

Spotlight

Advertisements