Issue 24, 2016

CRISPR/Cas9: a historical and chemical biology perspective of targeted genome engineering

Abstract

The CRISPR–Cas9 system has revolutionized the process of making changes to the DNA sequence of organisms. Relying on a simplistic model of RNA guided DNA binding and cleavage, this molecular toolbox has found application in nearly every branch of biological sciences. The story of CRISPR–Cas9 is one of discovery and development where a component of bacterial adaptive immunity has been harnessed to address important biological questions using significant inputs from physicochemical structure–function studies. In this review, we trace the evolution of CRISPR–Cas9 from its predecessor genome editing tools and document its current status with an emphasis on chemical biology aspects of modulating its activity to generate a potent tool for gene therapy applications.

Graphical abstract: CRISPR/Cas9: a historical and chemical biology perspective of targeted genome engineering

  • This article is part of the themed collection: Primer

Article information

Article type
Tutorial Review
Submitted
10 মার্চ 2016
First published
14 সেপ্টে. 2016

Chem. Soc. Rev., 2016,45, 6666-6684

CRISPR/Cas9: a historical and chemical biology perspective of targeted genome engineering

A. Singh, D. Chakraborty and S. Maiti, Chem. Soc. Rev., 2016, 45, 6666 DOI: 10.1039/C6CS00197A

To request permission to reproduce material from this article, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.

Social activity

Spotlight

Advertisements