Reaction-based indicator displacement assay (RIA) for the development of a triggered release system capable of biofilm inhibition†
Bethany L.
Patenall, 
‡a
George T.
Williams‡,
a
Lauren
Gwynne,
a
Liam J.
Stephens,
a
Emma V.
Lampard,
a
Hollie J.
Hathaway,
b
Amber E.
Young,
c
Mark J.
Sutton,
d
Robert D.
Short,
b
Steven D.
Bull,
a
Tony D.
James,
*a
Adam C.
Sedgwick
*e
and
A. Toby A.
Jenkins
*a
‡a
George T.
Williams‡,
a
Lauren
Gwynne,
a
Liam J.
Stephens,
a
Emma V.
Lampard,
a
Hollie J.
Hathaway,
b
Amber E.
Young,
c
Mark J.
Sutton,
d
Robert D.
Short,
b
Steven D.
Bull,
a
Tony D.
James,
*a
Adam C.
Sedgwick
*e
and
A. Toby A.
Jenkins
*a
Abstract
Here, a reaction-based indicator displacement hydrogel assay (RIA) was developed for the detection of hydrogen peroxide (H2O2) via the oxidative release of the optical reporter Alizarin Red S (ARS). In the presence of H2O2, the RIA system displayed potent biofilm inhibition for Methicillin-resistant Staphylococcus aureus (MRSA), as shown through an in vitro assay quantifying antimicrobial efficacy. This work demonstrated the potential of H2O2-responsive hydrogels containing a covalently bound diol-based drug for controlled drug release.
- This article is part of the themed collection: The Mechanics of Supramolecular Chemistry
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