Issue 4, 2018

Buffer-free integrative nanofluidic device for real-time continuous flow bioassays by ion concentration polarization

Abstract

To perform precision medicine in real-time, a sensor capable of continuously monitoring target biomolecules secreted from a patient under dynamic situations is essential. In this study, a novel portable device combining an aptamer probe and a nanofluidic component was developed, enabling the buffer-free continuous monitoring of small molecules in biological fluids. This integration is synergistic: the aptamer sensor is used to bind target biomolecules, triggering a fluorescence signal change, while the nanofluidic component is applied to achieve ion concentration polarization and convert serum into a clean buffer for aptamer signal regeneration. To demonstrate the system's versatility, we measured various adenosine triphosphate concentrations in human serum for hours with high sensitivity and specificity at minute temporal resolution. Our results demonstrate that this integrative device can be applied for the continuous measurement of target biomolecules and online signal regeneration in patient samples without the use of bulky clean buffer solutions for dynamic real-time healthcare.

Graphical abstract: Buffer-free integrative nanofluidic device for real-time continuous flow bioassays by ion concentration polarization

Supplementary files

Article information

Article type
Paper
Submitted
03 অক্টো. 2017
Accepted
20 ডিসে. 2017
First published
20 ডিসে. 2017

Lab Chip, 2018,18, 574-584

Buffer-free integrative nanofluidic device for real-time continuous flow bioassays by ion concentration polarization

D. Phan, L. Jin, S. Wustoni and C. Chen, Lab Chip, 2018, 18, 574 DOI: 10.1039/C7LC01066D

To request permission to reproduce material from this article, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.

Social activity

Spotlight

Advertisements