Issue 19, 2014

Nanotopography as a trigger for the microscale, autogenous and passive lysis of erythrocytes

Abstract

Microscale devices are increasingly being developed for diagnostic analysis although conventional lysis as an initial step presents limitations due to its scale or complexity. Here, we detail the physical response of erythrocytes to the surface nanoarchitecture of black Si (bSi) and foreshadow their potential in microanalysis. The physical interaction brought about by the spatial convergence of the two topologies: (a) the nanopillar array present on the bSi and (b) the erythrocyte cytoskeleton present on the red blood cells (RBCs), provides spontaneous stress-induced cell deformation, rupture and passive lysis within an elapsed time of ∼3 min from immobilisation to rupture and without external chemical or mechanical intervention. The mechano-responsive bSi surface provides highly active yet autogenous RBC lysis and a prospect as a front-end platform technology in evolving micro-fluidic platforms for cellular analyses.

Graphical abstract: Nanotopography as a trigger for the microscale, autogenous and passive lysis of erythrocytes

Supplementary files

Article information

Article type
Paper
Submitted
13 ফেব্রু. 2014
Accepted
13 মার্চ 2014
First published
14 মার্চ 2014

J. Mater. Chem. B, 2014,2, 2819-2826

Author version available

Nanotopography as a trigger for the microscale, autogenous and passive lysis of erythrocytes

V. T. H. Pham, V. K. Truong, D. E. Mainwaring, Y. Guo, V. A. Baulin, M. Al Kobaisi, G. Gervinskas, S. Juodkazis, W. R. Zeng, P. P. Doran, R. J. Crawford and E. P. Ivanova, J. Mater. Chem. B, 2014, 2, 2819 DOI: 10.1039/C4TB00239C

To request permission to reproduce material from this article, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.

Social activity

Spotlight

Advertisements