Issue 22, 2013

DNA-responsive disassembly of AuNP aggregates: influence of nonbase-paired regions and colorimetric DNA detection by exonuclease III aided amplification

Abstract

Due to great potential in nanobiotechnology, nanomachines, and smart materials, DNA-directed disassembly of gold nanoparticles (AuNPs) has been extensively explored. In a typical system, nonbase-paired regions (e.g., overhangs and gaps in the linker DNA and oligonucleotide spacers between thiol group and hybridization sequence) are indispensable portions in the disassembly of AuNPs based on DNA displacement reaction. Therefore, it is necessary to study the effect of nonbase-paired regions to improve the kinetics of disassembly of AuNPs. Herein, the disassembly rate of AuNPs based on DNA displacement reaction was investigated by using different length spacers and linker DNA containing various lengths of gaps or overhangs. Interestingly, it was revealed that among the gaps in the linker DNA could be most effectively used to improve the disassembly rate of the AuNPs. As a result, when we introduced gaps into linker DNA, the DNA displacement reaction of AuNPs was markedly shortened to less than 50 min, which was much faster than the previous methods. As a proof of the importance of our findings, a rapid AuNP-based colorimetric DNA biosensor has been successfully prepared. In addition, we showed that the signal of the biosensors could be further amplified using exonuclease III, resulting in a much lower detection limit in comparison with previous sensors similarly using AuNP aggregates as probes.

Graphical abstract: DNA-responsive disassembly of AuNP aggregates: influence of nonbase-paired regions and colorimetric DNA detection by exonuclease III aided amplification

Supplementary files

Article information

Article type
Paper
Submitted
12 ফেব্রু. 2013
Accepted
10 এপ্রিল 2013
First published
11 এপ্রিল 2013

J. Mater. Chem. B, 2013,1, 2851-2858

DNA-responsive disassembly of AuNP aggregates: influence of nonbase-paired regions and colorimetric DNA detection by exonuclease III aided amplification

Z. Zhou, W. Wei, Y. Zhang and S. Liu, J. Mater. Chem. B, 2013, 1, 2851 DOI: 10.1039/C3TB20206B

To request permission to reproduce material from this article, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.

Social activity

Spotlight

Advertisements