Issue 24, 2020

Enabling high-throughput single-animal gene-expression studies with molecular and micro-scale technologies

Abstract

Gene expression and regulation play diverse and important roles across all living systems. By quantifying the expression, whether in a sample of single cells, a specific tissue, or in a whole animal, one can gain insights into the underlying biology. Many biological questions now require single-animal and tissue-specific resolution, such as why individuals, even within an isogenic population, have variations in development and aging across different tissues and organs. The popular techniques that quantify the transcriptome (e.g. RNA-sequencing) process populations of animals and cells together and thus, have limitations in both individual and spatial resolution. There are single-animal assays available (e.g. fluorescent reporters); however, they suffer other technical bottlenecks, such as a lack of robust sample-handling methods. Microfluidic technologies have demonstrated various improvements throughout the years, and it is likely they can enhance the impact of these single-animal gene-expression assays. In this perspective, we aim to highlight how the engineering/method-development field have unique opportunities to create new tools that can enable us to robustly answer the next set of important questions in biology that require high-density, high-quality gene expression data.

Graphical abstract: Enabling high-throughput single-animal gene-expression studies with molecular and micro-scale technologies

Article information

Article type
Perspective
Submitted
01 сеп 2020
Accepted
29 окт 2020
First published
30 окт 2020

Lab Chip, 2020,20, 4528-4538

Author version available

Enabling high-throughput single-animal gene-expression studies with molecular and micro-scale technologies

J. Wan and H. Lu, Lab Chip, 2020, 20, 4528 DOI: 10.1039/D0LC00881H

To request permission to reproduce material from this article, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.

Social activity

Spotlight

Advertisements