Issue 19, 2017

Measuring macromolecular crowding in cells through fluorescence anisotropy imaging with an AIE fluorogen

Abstract

We report a new strategy that allows spatiotemporal visualization of the macromolecular crowding effect in cells. An amine-reactive aggregation-induced emission fluorogen is used to label proteins in the cytoplasm and the change in the protein mobility as well as local viscosity can be monitored by using fluorescence anisotropy imaging and fluorescence lifetime imaging, respectively.

Graphical abstract: Measuring macromolecular crowding in cells through fluorescence anisotropy imaging with an AIE fluorogen

Supplementary files

Article information

Article type
Communication
Submitted
14 дек 2016
Accepted
13 фев 2017
First published
13 фев 2017

Chem. Commun., 2017,53, 2874-2877

Measuring macromolecular crowding in cells through fluorescence anisotropy imaging with an AIE fluorogen

H. Soleimaninejad, M. Z. Chen, X. Lou, T. A. Smith and Y. Hong, Chem. Commun., 2017, 53, 2874 DOI: 10.1039/C6CC09916E

To request permission to reproduce material from this article, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.

Social activity

Spotlight

Advertisements