Issue 47, 2023

Covalent crosslinking in gas-phase biomolecular ions. An account and perspective

Abstract

Photochemical crosslinking in gas-phase ion complexes has been introduced as a method to study biomolecular structures and dynamics. Emphasis has been on carbene-based crosslinking induced by photodissociation of diazirine-tagged ions. The features that characterize gas-phase crosslinking include (1) complex formation in electrospray droplets that allows for library-type screening; (2) well defined stoichiometry of the complexes due to mass-selective isolation; (3) facile reaction monitoring and yield determination, and (4) post-crosslinking structure analysis by tandem mass spectrometry that has been combined with hydrogen–deuterium exchange, UV-vis action spectroscopy, and ion mobility measurements. In this account, examples are given of peptide–peptide, peptide–nucleotide, and peptide–ligand crosslinking that chiefly used carbene-based reactions. The pros and cons of gas-phase crosslinking are discussed. Nitrile-imine based crosslinking in gas-phase ions is introduced as a promising new approach to ion structure analysis that offers high efficiency and has the potential for wide ranging applications.

Graphical abstract: Covalent crosslinking in gas-phase biomolecular ions. An account and perspective

Article information

Article type
Perspective
Submitted
08 окт 2023
Accepted
09 ное 2023
First published
13 ное 2023

Phys. Chem. Chem. Phys., 2023,25, 32292-32304

Covalent crosslinking in gas-phase biomolecular ions. An account and perspective

F. Tureček, Phys. Chem. Chem. Phys., 2023, 25, 32292 DOI: 10.1039/D3CP04879A

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