Volume 205, 2017

Real-time dynamic SERS detection of galectin using glycan-decorated gold nanoparticles

Abstract

We present the application of surface-enhanced Raman scattering (SERS) spectroscopy for the fast, sensitive and highly specific detection of the galectin-9 (Gal-9) protein in binding buffer (mimicking natural conditions). The method involves the use of specifically designed nanotags comprising glycan-decorated gold nanoparticles encoded with 4-mercaptobenzoic acid. At fast time scales Gal-9 can be detected down to a concentration of 1.2 nM by monitoring the SERS signal of the reporter, driven by aggregation of the functionalized Au NPs tags, induced by Gal-9 recognition. We additionally demonstrate that the sensitivity and concentration working range of the sensor can be tuned via control of aggregation dynamics and cluster size distribution.

Associated articles

Supplementary files

Article information

Article type
Paper
Submitted
07 апр 2017
Accepted
04 май 2017
First published
04 май 2017

Faraday Discuss., 2017,205, 363-375

Real-time dynamic SERS detection of galectin using glycan-decorated gold nanoparticles

J. Langer, I. García and L. M. Liz-Marzán, Faraday Discuss., 2017, 205, 363 DOI: 10.1039/C7FD00123A

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