High-resolution complementary chemical imaging of bio-elements in Caenorhabditis elegans

Dominic J. Hare; Michael W. M. Jones; Verena C. Wimmer; Nicole L. Jenkins; Martin D. de Jonge; Ashley I. Bush; Gawain McColl

doi:10.1039/C5MT00288E

High-resolution complementary chemical imaging of bio-elements in Caenorhabditis elegans†

Dominic J. Hare,

‡^ab Michael W. M. Jones,‡^cd Verena C. Wimmer,^b Nicole L. Jenkins,^b Martin D. de Jonge,^c Ashley I. Bush

^b and Gawain McColl*^b

Author affiliations

* Corresponding authors

^a Elemental Bio-imaging Facility, University of Technology Sydney, Broadway, New South Wales, Australia

^b The Florey Institute of Neuroscience and Mental Health, University of Melbourne, Parkville, Victoria, Australia
E-mail: gmccoll@florey.edu.au
Tel: +61 3 9035 6608

^c Australian Synchrotron, Clayton, Victoria, Australia

^d ARC Centre of Excellence in Advanced Molecular Imaging, La Trobe Institute for Molecular Sciences, La Trobe University, Melbourne, Australia

Abstract

Here, we present a sub-μm multimodal approach to image essential elements in Caenorhabditis elegans. A combination of chemical imaging technologies reveals total metal concentration, chemical state and the protein to which an element is associated. This application of distinct yet complementary chemical imaging techniques provided unique insight into essential and trace elements at the subcellular level.

This article is part of the themed collection: Metallomics Recent HOT articles

Metallomics

High-resolution complementary chemical imaging of bio-elements in Caenorhabditis elegans†

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