Assessing the impact of food-derived bioactives on digestive proteases by in vitro and in silico approaches

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From static to semi-dynamic in vitro digestion conditions relevant for the older population: starch and protein digestion of cooked lentils

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Food Funct., 2024, 15, 591
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In vitro digestion of two protein-rich dairy products in the ageing gastrointestinal tract

Anaïs Lavoisier, Martine Morzel, Séverine Chevalier, Gwénaële Henry, Julien Jardin, Marielle Harel-Oger, Gilles Garric and Didier Dupont

Food Funct., 2023, 14, 9377
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Antioxidant peptides of sheep placental extract digestion product: In vitro and in silico study

Ahmidin Wali, Naziermu Dongmulati, Gulmire Turdu, Ailin Hu, Han He, Xinmin Zhao, Yanhua Gao, Abulimiti Yili, Hajiakber Aisa and Shavkat Salikhov

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Impact of the stepwise implementation of INFOGEST semi-dynamic conditions on in vitro starch and protein digestion: A case study on lentil cotyledon cells

D. Duijsens, S.H.E. Verkempinck and T. Grauwet

Food Research International, 2024, 197, 115214
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Kinetic description of changes in the size of casein microparticles under simulated gastric conditions

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A Multi-Enzyme Cascade Response for the Colorimetric Recognition of Organophosphorus Pesticides Utilizing Core-Shell Pd@Pt Nanoparticles with High Peroxidase-like Activity

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Foods, 2023, 12, 3319
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Effect of the combined addition of hyaluronic acid and gellan gum on the physicochemical and digestive properties of tofu

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Exercise-Induced Allergic Reactions in Children Desensitized to Hen’s Eggs and Cow’s Milk by Oral Immunotherapy

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The Journal of Allergy and Clinical Immunology: In Practice, 2023, 11, 3187
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Construction of Probiotic Double-Layered Multinucleated Microcapsules Based on Sulfhydryl-Modified Carboxymethyl Cellulose Sodium for Increased Intestinal Adhesion of Probiotics and Therapy for Intestinal Inflammation Induced by Escherichia coli O157:H7

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ACS Appl. Mater. Interfaces, 2023, 15, 18569
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Interactions between polystyrene nanoplastics and bovine lactoferrin in simulated gastric fluids: Aggregation kinetics and impact on protein digestion

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Journal of Hazardous Materials, 2026, 501, 140820
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Interplay of egg white gel pH and intragastric pH: Impact on breakdown kinetics and mass transport processes

Alisha Kar, Alex G. Olenskyj, Maria Garcia Guerrero, Riley Graham and Gail M. Bornhorst

Food Research International, 2023, 173, 113290
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Phytochemical composition, in vitro simulated gastric and intestinal digestion, cytotoxicity and molecular docking of ethno-polyherbal formulation used in diabetes management

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Bioactive Peptides from Bovine Colostrum: Sources, Structures, and Biomedical Applications

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Oxidation-mediated structure and molecular interaction transformation of egg white protein: The underlying mechanism of functional properties and in vitro gastric digestibility improvement

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Food Chemistry, 2023, 405, 134874
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A tool for predicting pH and temperature effects on porcine and human pepsin activity during in vitro gastric digestion

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Sci Rep, 2026, 16
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Gastroesophageal reflux-induced cough: the positive feedback loop of microaspiration and neurogenic inflammation

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Protein digestion and absorption: the influence of food processing

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Peptide profiling and antioxidant characterization of the simulated gastrointestinal digest of hemp seed proteins

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Physicochemical characteristics and nutritional values of Javanese grasshopper (Valanga nigricornis Burm.) flour

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Overcoming Challenges in the Metabolism of Peptide Therapeutics: Strategies and Case Studies for Clinical Success

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Correlating structure and activity of pepsin enzyme in H2O and D2O for the study of gastric digestion

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Liquid-like Oral Sustained-Release System Based on Acid-Sensitive in situ Hydrogel for Alleviate Gastrointestinal Side Effects of Indobufen

Ya Tang, Peng Sun, Adric Hii Ru Khiing, Kang Sha, Xiaole Qi and Zhenghong Wu

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In vitro gastrointestinal digestibility under infant conditions of bovine milk fat emulsions stabilized using decalcified micellar casein concentrate

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Research on the process of synergistic degradation of corn straw by probiotics—enzymes based on microbiome and metabolomics

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In vitro gastrointestinal simulation digestion, absorption and bioavailability of four animal-based proteins in canine and feline food

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Characteristics of Crude Pepsin Enzyme from Catfish Stomach (Clarias sp.)

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Determination of pepsin in human saliva using pepsin-susceptible peptide reporter and colorimetric dipstick assay: a prospective, cross-sectional study

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Microchim Acta, 2024, 191
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In vitro digestion and functional properties of bovine β-casein: A comparison between adults and infants

Xiaoqian Chen, Rui Fan, Xinyu Wang, Lina Zhang, Caiyun Wang, Zhanqun Hou, Chun Li, Libo Liu and Jian He

Food Research International, 2024, 194, 114914
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Tea, coffee, and cocoa delay milk gastric coagulation in a biomimetic in vitro system (NERDT™) by hindering both acid- and pepsin-induced milk gelation

Isuri A. Jayawardana, Amélie Deglaire, Olivia Ménard, Marie-Francoise Cochet, Yann Le Gouar, Jordane Ossemond, Vincent Mathieu, Martine Morzel, Didier Dupont and Steven Le Feunteun

Food Research International, 2026, 233, 119033
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Antioxidant and Antibacterial Potential of Pepsin-Hydrolyzed Bioactive Peptides from IPB D1 Chicken Egg White

T.M. Alfiansyah and Z. Wulandari

IOP Conf. Ser.: Earth Environ. Sci., 2025, 1484, 012024
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Computational characteristics of kinetic models for in vitro protein digestion: A review

Peter Adeoye Sopade

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Microalga Nannochloropsis gaditana as a Sustainable Source of Bioactive Peptides: A Proteomic and In Silico Approach

Samuel Paterson, Laura Alonso-Pintre, Esperanza Morato-López, Sandra González de la Fuente, Pilar Gómez-Cortés and Blanca Hernández-Ledesma

Foods, 2025, 14, 252
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Non-covalent interactions of roselle anthocyanins with milk proteins and egg white protein

Ying Wang, Songen Wang, Xuan Zhang, Wenhui Wu, Weibin Bai and Lingmin Tian

Food Hydrocolloids, 2024, 154, 110125
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Applying RSM for optimization of Ferula assafoetida protein hydrolysis to enhance bioactivity and functional properties

Ali Akbar Gholami, Mohammad Sadegh Taghizadeh, Alireza Afsharifar, Ali Moghadam, Zohreh-Sadat Mortazavi and Ali Niazi

Applied Food Research, 2025, 5, 100915
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Exploratory data analysis of the correlation between bioactive components in various tea extracts and the digestive function in vitro

Kang-Woo Lee, In-Su Na, Dong-Ho Lee, Jin-Woo Kim, Na-Yeon Lee, Jin-Oh Chung, Hyun Woo Jeong, Jonghee Sohn, Wan-Gi Kim and Soon-Mi Shim

Food Bioscience, 2024, 60, 104404
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Milk protein-based cryogel monoliths as novel encapsulants of probiotic bacteria. Part II: Lacticaseibacillus rhamnosus GG storage stability and bioactivity under in vitro digestion

Thierry Hellebois, Romain Canuel, Céline C. Leclercq, Claire Gaiani and Christos Soukoulis

Food Hydrocolloids, 2024, 146, 109173
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The Modification of Coffee Beans Through a Combination of Microbial and Enzymatic Processes

Paulina Pakosz, Anna Bzducha-Wróbel, Beata Drużyńska and Rafał Wołosiak

Foods, 2026, 15, 859
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Compound probiotics microcapsules improve milk yield and milk quality of dairy cows by regulating intestinal flora

Rina Wu, Shihan Chang, Henan Zhang, Xujing Yang, Ruixia Gu, Shuo Wang, Xiaoyu Liu, Xiaoyan Liu, Munkhdelger Bat‐Ochir and Junrui Wu

Food Bioengineering, 2024, 3, 110
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pH-dependent effects of pepsin and trypsin on the stability and antibiofilm functionality of pea protein–stabilized carvacrol nanoemulsions

Jun Ji, Mohamed Brahmi, Emilie Dumas, Nour-Eddine Chihib and Adem Gharsallaoui

Food Chemistry: X, 2026, 33, 103450
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Sex-based differences in in vitro digestibility of milk and oat drink, and powder counterparts

Leehen Mashiah, Eden Beck, Carmit Shani Levi and Uri Lesmes

Food Research International, 2025, 214, 116610
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Quantitative peptide release kinetics to describe the effect of pH on pepsin preference

Gijs J.C. Vreeke, Jean-Paul Vincken and Peter A. Wierenga

Process Biochemistry, 2023, 134, 351
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Alginate binding enhances the structural stability and potentiates the lytic activity of bacteriophage endolysin's partially folded conformation

Mayur Mohan Ghate, Khushboo Gulati and Krishna Mohan Poluri

Archives of Biochemistry and Biophysics, 2024, 760, 110129
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Spatial-temporal mapping of the intra-gastric pepsin concentration and proteolysis in pigs fed egg white gels

Françoise Nau, Steven Le Feunteun, Yann Le Gouar, Gwénaële Henry, Maryvonne Pasco, Catherine Guérin-Dubiard, Kéra Nyemb-Diop and Didier Dupont

Food Chemistry, 2022, 389, 133132
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Nutritional management of survivors of critical illness: The clinical pharmacists' role

Zenzi Rosseel, Stephanie C.M. Wuyts, Marjoleine Desmedt, Sandrina von Winckelmann, Elisabeth De Waele, Claudine Ligneel and Pieter-Jan Cortoos

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Feasibility of using a realistic food bolus for semi-dynamic in vitro gastric digestion of hard cheese with pH-stat monitoring of protein hydrolysis

Martine Morzel, Sylvaine Ramsamy and Steven Le Feunteun

Food Research International, 2023, 169, 112818
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Designing Gastric-Stable Adsorption Layers by Whey Protein–Pectin Complexation at the Oil–Water Interface

Hao Li and Paul Van der Meeren

J. Agric. Food Chem., 2023, 71, 7109
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Studying the Process of Enzyme Treatment on Beef Meat-Bone Paste Quality

Assemgul Baikadamova, Aitbek Kakimov, Zhanibek Yessimbekov, Anuarbek Suychinov, Rasul Turagulov, Duman Orynbekov, Gulmira Zhumadilova and Yerlan Zharykbasov

Applied Sciences, 2024, 14, 8703
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Digestion of food proteins: the role of pepsin

Mengxiao Yang, Zhi Yang, David W. Everett, Elliot Paul Gilbert, Harjinder Singh and Aiqian Ye

Critical Reviews in Food Science and Nutrition, 2025, 65, 6919
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Antosiyaninlerin Gıda Matrisindeki Diğer Bileşenlerle Etkileşimlerinin Antosiyaninlerin Sindirim ve Emilimlerinin Üzerine Etkileri

Melisa Kırnapcı and Müzeyyen Berkel Kaşıkçı

Uşak Üniversitesi Fen ve Doğa Bilimleri Dergisi, 2025, 9, 144
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Impact of aging on the digestive system related to protein digestion in vivo

Fenna Hinssen, Marco Mensink, Thom Huppertz and Nikkie van der Wielen

Critical Reviews in Food Science and Nutrition, 2025, 65, 5829
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Decalcification strongly affects in vitro gastrointestinal digestion of bovine casein micelles under infant, adult and elderly conditions

Keyu Wang, Dasong Liu, Xiumei Tao, Jie Zhang, Thom Huppertz, Joe M. Regenstein, Xiaoming Liu and Peng Zhou

Food Hydrocolloids, 2023, 139, 108515
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Variations in Bovine Milk Proteins and Processing Conditions and Their Effect on Protein Digestibility in Humans: A Review of In Vivo and In Vitro Studies

Conor J. Fitzpatrick, Daniela Freitas, Tom F. O’Callaghan, James A. O’Mahony and André Brodkorb

Foods, 2024, 13, 3683
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Influence of food macrostructure on the kinetics of acidification in the pig stomach after the consumption of rice- and wheat-based foods: Implications for starch hydrolysis and starch emptying rate

Joanna Nadia, Alexander G. Olenskyj, Parthasarathi Subramanian, Suzanne Hodgkinson, Natascha Stroebinger, Talia G. Estevez, R. Paul Singh, Harjinder Singh and Gail M. Bornhorst

Food Chemistry, 2022, 394, 133410
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Quantitative magnetic resonance imaging of in vitro gastrointestinal digestion of a bread and cheese meal

Maja Musse, Steven Le Feunteun, Guylaine Collewet, Mattéi Ravilly, Stéphane Quellec, Jordane Ossemond, Martine Morzel, Sylvain Challois, Françoise Nau and Tiphaine Lucas

Food Research International, 2023, 169, 112821
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Enzymatic dephosphorylation of isolated bovine caseins: Targeted modulation of phosphorylation degree via pH and reaction time optimization

Jing Che, Wanxiang Guo, Nina Aagaard Poulsen and Lotte Bach Larsen

Food Bioscience, 2025, 69, 106855
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Promising bioactive peptides from turkey cruor: How hydrolysis duration and pH could affect the antimicrobial activity and peptide population

Houssine Fliss, Jacinthe Thibodeau, Ismail Fliss, Laurent Bazinet and Sergey Mikhaylin

Food Bioscience, 2025, 71, 106983
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Monitoring the effect of consumption temperature of full-fat milk on in vitro gastric digestion using Magnetic Resonance Imaging

Conor J. Fitzpatrick, Maja Musse, Jiajun Feng, Guylaine Collewet, Tiphaine Lucas, Mark Timlin, Sylvain Challois, Stephane Quellec, Didier Dupont, André Brodkorb, Daniela Freitas and Steven Le Feunteun

Food Hydrocolloids, 2024, 152, 109864
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Decellularized amnion membrane-based thermosensitive vaginal hydrogel enhances ritodrine efficacy and reduces systemic side effects in preterm birth treatment

Yu Xin, Yue Chen, Xiaojun Zhu, Ying Zhang, Maiqi Kong, Huidi Jiang, Xiao Li, Weidong Fei and Caihong Zheng

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Protein@AuNPs Synthesized by Microfluidic Droplet System and Application in Cu(II) and L-Cysteine Sensing

Xiaotong Zhu, Xinyu Li, Li Qi, Rongyue Zhang, Nan Li, Xiaonan He and Juan Qiao

Chem. Res. Chin. Univ., 2025, 41, 131
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Casein structures differently affect postprandial amino acid delivery through their intra-gastric clotting properties

Audrey Boulier, Sylvain Denis, Gwénaële Henry, Sylvie Guérin, Monique Alric, Nathalie Meunier, Adeline Blot, Bruno Pereira, Corinne Malpuech-Brugere, Didier Remond, Yves Boirie, Alain Baniel, Ruddy Richard, Didier Dupont and Gaëlle Boudry

Food Chemistry, 2023, 415, 135779
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In vitro protein and starch digestibilities of multigrain sorghum-barley mixtures

Xinglin Jin, Yilang Li, Su Sin Koa and Peter Adeoye Sopade

Journal of Food Engineering, 2024, 379, 112135
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Proteomic Profiling and Cytotoxic Effects of Short-Chain Protein Hydrolysates from Bacillus siamensis MH3-Fermented Soybean Meal on Colon Cancer Cells

Siranat Tantasirin, Duenrut Chonudomkul, Wannarat Phonphoem, Onrapak Reamtong and Nattanan Panjaworayan T-Thienprasert

ACS Food Sci. Technol., 2025, 5, 4363
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Experimental Protocols Used to Mimic Gastrointestinal Protein Digestion: A Systematic Review

Anna Beatriz Santana Luz, Amanda Fernandes de Medeiros, Gidyenne Christine Bandeira Silva de Medeiros, Grasiela Piuvezam, Thaís Souza Passos and Ana Heloneida de Araújo Morais

Nutrients, 2024, 16, 2398
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Gastric devolution of transglutaminase-induced acid and rennet-induced casein gels using dynamic DIDGI® and static COST action INFOGEST protocols

Amy Logan, Olivia Ménard, Meltem Bayrak, Elham Rakhshi and Juliane Floury

Food Research International, 2023, 164, 112351
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Stimulation of Acanthamoeba castellanii excystment by enzyme treatment and consequences on trophozoite growth

Zineb Fechtali-Moute, Philippe M. Loiseau and Sébastien Pomel

Front. Cell Dev. Biol., 2022, 10
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High-Protein Diets: Characteristics of Bacterial Fermentation and Its Consequences on Intestinal Health

Fatima Omer, Xin Song, Enting Qiao, Xuezhao Sun, Hao Zhang, Mengzhi Wang and Yujia Jing

Fermentation, 2025, 11, 678
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Effect of consumption temperature of whole milk on in vitro gastric digestion using a biomimetic digestion simulator

Jiajun Feng, Jeehyun Lee, Vincent Mathieu, Yann Le Gouar, Romain Jeantet, Didier Dupont and Steven Le Feunteun

Food Research International, 2026, 223, 117869
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Isomerization and bioaccessibility of cypermethrin and fenpropathrin in Pacific oyster during simulated digestion as influenced by domestic cooking methods

Hangtao Xie, Yadan Jiao, Tian Li, Tuo Zhang, Yanyan Zheng, Yongkang Luo, Yuqing Tan, Chune Liu and Hui Hong

FIA, 2023, 2, 9
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Advances in Understanding the Antioxidant and Antigenic Properties of Egg-Derived Peptides

Mihaela Brumă (Călin), Ina Vasilean, Leontina Grigore-Gurgu, Iuliana Banu and Iuliana Aprodu

Molecules, 2024, 29, 1327
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Unveiling the significance of gastric digestion in gastrointestinal fate of octenylsuccinylated starch-stabilized emulsions

Myeongsu Jo and Yong-Cheng Shi

Carbohydrate Polymers, 2024, 333, 121953
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Papain-Based Solubilization of Decellularized Extracellular Matrix for the Preparation of Bioactive, Thermosensitive Pregels

Ahed Almalla, Laura Elomaa, Leïla Bechtella, Assal Daneshgar, Prabhu Yavvari, Zeinab Mahfouz, Peter Tang, Beate Koksch, Igor Sauer, Kevin Pagel, Karl Herbert Hillebrandt and Marie Weinhart

Biomacromolecules, 2023, 24, 5620
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Combined Effect of Drying Methods and Enzymatic Hydrolysis on Antioxidant Activity, ACE-Inhibitory and Functional Properties of Black Garlic

Farouq Heidar Barido, Kok-Gan Chan, Tirtadanu, Kah Ooi Chua, Teguh Wahyono, Dicky Tri Utama and Sung Ki Lee

Food Biophysics, 2026, 21
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Impact of age on the digestion of cream cheese formulated with opposite caseins to whey proteins ratios: An in vitro study

Anaïs Lavoisier, Séverine Chevalier, Gwénaële Henry, Jordane Ossemond, Marielle Harel-Oger, Gilles Garric, Didier Dupont and Martine Morzel

Food Research International, 2024, 190, 114621
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New In Vitro Method Complement Low-value Existing In Vitro Method in Commercial Dog Diets in the Republic of Korea

Kyeong Ho Jeon, Min Ho Song, Ji Hwan Lee, Se Yeon Chang, Dong Cheol Song, Jae Woo An, Se Hyun Park, Hyuck Kim and Jin Ho Cho

J. Agirc. Life Sci., 2024, 58, 101
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Intragastric restructuring dictates the digestive kinetics of heat-set milk protein gels of contrasting textures

Siqi Li, Tanyaradzwa Mungure, Aiqian Ye, Simon M. Loveday, Ashling Ellis, Mike Weeks and Harjinder Singh

Food Research International, 2024, 195, 114944
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In vitro gastrointestinal digestion of cow’s and sheep’s dairy products: Impact of species and structure

Tanguy Saviard, Olivia Menard, Stefano Nebbia, Jordane Ossemond, Gwénaële Henry, Raphaël Chacon, Steven Le Feunteun, Didier Dupont and Linda Le Roux

Food Research International, 2024, 190, 114604
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An improved, filtration-based process to purify functional mucins from mucosal tissues with high yields

Matthias Marczynski, Carolin A. Rickert, Tobias Fuhrmann and Oliver Lieleg

Separation and Purification Technology, 2022, 294, 121209
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The Effects of a Microbial Enzyme Mixture on Macronutrient Hydrolysis in a Static Simulation of Oro-Gastric Digestion That Models Human Digestive Senescence

Sean M. Garvey, Erin N. Madden, Yunyao Qu, Caroline H. Best and Kelly M. Tinker

Foods, 2025, 14, 937
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First assessments of nutrient bioaccessibility with an INFOGEST semi-dynamic gastric digestion in vitro protocol adapted to model proton pump inhibitor use

Ruoxuan Deng, Françoise Nau, Tiphaine Lucas, Marie-Bernadette Maillard, Arlette Leduc, Jordane Ossemond, Maja Musse and Steven Le Feunteun

Food Research International, 2025, 203, 115833
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Invited review: Camel milk and gut health—Understanding digestibility and the effect on gut microbiota

Abdelmoneim H. Ali, Siqi Li, Shao-Quan Liu, Ren-You Gan, Hua-Bin Li, Afaf Kamal-Eldin and Mutamed Ayyash

Journal of Dairy Science, 2024, 107, 2573
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