Issue 6, 2023

The potential role of serum extracellular vesicle derived small RNAs in AML research as non-invasive biomarker

Abstract

Background: Extracellular vesicles (EV) are cell-derived vesicles released by all cells in health and disease. Accordingly, EVs are also released by cells in acute myeloid leukemia (AML), a hematologic malignancy characterized by uncontrolled growth of immature myeloid cells, and these EVs likely carry markers and molecular cargo reflecting the malignant transformation occurring in diseased cells. Monitoring antileukemic or proleukemic processes during disease development and treatment is essential. Therefore, EVs and EV-derived microRNA (miRNA) from AML samples were explored as biomarkers to distinguish disease-related patterns ex vivo or in vivo. Methodology: EVs were purified from serum of healthy (H) volunteers and AML patients by immunoaffinity. EV surface protein profiles were analyzed by multiplex bead-based flow cytometry (MBFCM) and total RNA was isolated from EVs prior to miRNA profiling via small RNA sequencing. Results: MBFCM revealed different surface protein patterns in H versus AML EVs. miRNA analysis showed individual as well as highly dysregulated patterns in H and AML samples. Conclusions: In this study, we provide a proof-of-concept for the discriminative potential of EV derived miRNA profiles as biomarkers in H versus AML samples.

Graphical abstract: The potential role of serum extracellular vesicle derived small RNAs in AML research as non-invasive biomarker

Supplementary files

Article information

Article type
Paper
Submitted
30 дек 2022
Accepted
13 фев 2023
First published
20 фев 2023
This article is Open Access
Creative Commons BY-NC license

Nanoscale Adv., 2023,5, 1691-1705

The potential role of serum extracellular vesicle derived small RNAs in AML research as non-invasive biomarker

L. Li, V. Mussack, A. Görgens, E. Pepeldjiyska, A. S. Hartz, H. Aslan, E. Rackl, A. Rank, J. Schmohl, S. El Andaloussi, M. W. Pfaffl and H. Schmetzer, Nanoscale Adv., 2023, 5, 1691 DOI: 10.1039/D2NA00959E

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