Issue 39, 2016

Designing a biostable L-DNAzyme for lead(ii) ion detection in practical samples

Abstract

A promising biosensor for effective lead(II) ion detection in practical applications was developed by constructing a Pb2+-specific L-DNAzyme, the enantiomer of the natural nucleic acid-constructed D-DNAzyme. This fluorescence sensor contains the L-enzyme strand with a quencher at the 3′ end, and the L-substrate strand with a fluorophore at the 5′ and a quencher at the 3′ ends that formed a complex. In the presence of Pb2+, the L-substrate is cut into two fragments, leading to the recovery of fluorescence. The sensor shows high sensitivity and selectivity for Pb2+ detection with a linear response in the range of 5–100 nM and a detection limit of 3 nM in aqueous solution. Importantly, based on the fact that L-DNAzyme consists of non-natural nucleic acids, which are insensitive to nuclease digestion, protein adsorption and D-DNA hybridization, our sensor shows a specific response to Pb2+ in practical water and serum samples. Therefore, it is expected that our L-DNAzyme-based strategy may offer a new method for developing simple, rapid and sensitive sensors in complex systems.

Graphical abstract: Designing a biostable L-DNAzyme for lead(ii) ion detection in practical samples

Supplementary files

Article information

Article type
Paper
Submitted
23 июн 2016
Accepted
05 сен 2016
First published
06 сен 2016

Anal. Methods, 2016,8, 7260-7264

Designing a biostable L-DNAzyme for lead(II) ion detection in practical samples

H. Liang, S. Xie, L. Cui, C. Wu and X. Zhang, Anal. Methods, 2016, 8, 7260 DOI: 10.1039/C6AY01791F

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