Issue 5, 2016

Target identification of natural products and bioactive compounds using affinity-based probes

Abstract

Covering: 2010 to 2014.

Advances in isolation, synthesis and screening strategies have made many bioactive substances available. However, in most cases their putative biological targets remain unknown. Herein, we highlight recent advances in target identification of natural products and bioactive compounds by using affinity-based probes. Aided by photoaffinity labelling, this strategy can capture potential cellular targets (on and off) of a natural product or bioactive compound in live cells directly, even when the compound–target interaction is reversible with moderate affinity. The knowledge of these targets may help uncover molecular pathways and new therapeutics for currently untreatable diseases. In this highlight, we will introduce the development of various photoactivatable groups, their synthesis and applications in target identification of natural products and bioactive compounds, with a focus on work done in recent years and from our laboratory. We will further discuss the strengths and weaknesses of each group and the outlooks for this novel proteome-wide profiling strategy.

Graphical abstract: Target identification of natural products and bioactive compounds using affinity-based probes

Article information

Article type
Highlight
Submitted
24 avq 2015
First published
18 noy 2015

Nat. Prod. Rep., 2016,33, 612-620

Author version available

Target identification of natural products and bioactive compounds using affinity-based probes

S. Pan, H. Zhang, C. Wang, S. C. L. Yao and S. Q. Yao, Nat. Prod. Rep., 2016, 33, 612 DOI: 10.1039/C5NP00101C

To request permission to reproduce material from this article, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.

Social activity

Spotlight

Advertisements