Issue 8, 2016

3D printed nervous system on a chip

Abstract

Bioinspired organ-level in vitro platforms are emerging as effective technologies for fundamental research, drug discovery, and personalized healthcare. In particular, models for nervous system research are especially important, due to the complexity of neurological phenomena and challenges associated with developing targeted treatment of neurological disorders. Here we introduce an additive manufacturing-based approach in the form of a bioinspired, customizable 3D printed nervous system on a chip (3DNSC) for the study of viral infection in the nervous system. Micro-extrusion 3D printing strategies enabled the assembly of biomimetic scaffold components (microchannels and compartmented chambers) for the alignment of axonal networks and spatial organization of cellular components. Physiologically relevant studies of nervous system infection using the multiscale biomimetic device demonstrated the functionality of the in vitro platform. We found that Schwann cells participate in axon-to-cell viral spread but appear refractory to infection, exhibiting a multiplicity of infection (MOI) of 1.4 genomes per cell. These results suggest that 3D printing is a valuable approach for the prototyping of a customized model nervous system on a chip technology.

Graphical abstract: 3D printed nervous system on a chip

  • This article is part of the themed collection: 3D Printing

Associated articles

Supplementary files

Article information

Article type
Paper
Submitted
14 okt 2015
Accepted
08 dek 2015
First published
10 dek 2015

Lab Chip, 2016,16, 1393-1400

Author version available

3D printed nervous system on a chip

B. N. Johnson, K. Z. Lancaster, I. B. Hogue, F. Meng, Y. L. Kong, L. W. Enquist and M. C. McAlpine, Lab Chip, 2016, 16, 1393 DOI: 10.1039/C5LC01270H

To request permission to reproduce material from this article, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.

Social activity

Spotlight

Advertisements