Issue 10, 2015

A library approach to rapidly discover photoaffinity probes of the mRNA decapping scavenger enzyme DcpS

Abstract

Despite its diverse applications, such as identification of the protein binding partners of small molecules and investigation of intracellular drug–target engagement, photoaffinity labelling (PAL) is intrinsically challenging, primarily due to the difficulty in discovering functionally active photoaffinity probes. Here we describe the creation of a chemoproteomic library to discover a novel photoaffinity probe for DcpS, an mRNA decapping enzyme that is a putative target for Spinal Muscular Atrophy. This library approach expedites the discovery of photoaffinity probes and expands the chemical biology toolbox to include RNA cap-binding proteins.

Graphical abstract: A library approach to rapidly discover photoaffinity probes of the mRNA decapping scavenger enzyme DcpS

Supplementary files

Article information

Article type
Communication
Submitted
23 apr 2015
Accepted
05 may 2015
First published
05 may 2015

Mol. BioSyst., 2015,11, 2709-2712

Author version available

A library approach to rapidly discover photoaffinity probes of the mRNA decapping scavenger enzyme DcpS

H. Xu, E. C. Hett, A. Gopalsamy, M. D. Parikh, K. F. Geoghegan, R. E. Kyne, C. A. Menard, A. Narayanan, R. P. Robinson, D. S. Johnson, M. A. Tones and L. H. Jones, Mol. BioSyst., 2015, 11, 2709 DOI: 10.1039/C5MB00288E

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