Issue 5, 2023

Exploring multivalent carbohydrate–protein interactions by NMR

Abstract

Nuclear Magnetic Resonance (NMR) has been widely employed to assess diverse features of glycan–protein molecular recognition events. Different types of qualitative and quantitative information at different degrees of resolution and complexity can be extracted from the proper application of the available NMR-techniques. In fact, affinity, structural, kinetic, conformational, and dynamic characteristics of the binding process are available. Nevertheless, except in particular cases, the affinity of lectin-sugar interactions is weak, mostly at the low mM range. This feature is overcome in biological processes by using multivalency, thus augmenting the strength of the binding. However, the application of NMR methods to monitor multivalent lectin–glycan interactions is intrinsically challenging. It is well known that when large macromolecular complexes are formed, the NMR signals disappear from the NMR spectrum, due to the existence of fast transverse relaxation, related to the large size and exchange features. Indeed, at the heart of the molecular recognition event, the associated free-bound chemical exchange process for both partners takes place in a particular timescale. Thus, these factors have to be considered and overcome. In this review article, we have distinguished, in a subjective manner, the existence of multivalent presentations in the glycan or in the lectin. From the glycan perspective, we have also considered whether multiple epitopes of a given ligand are presented in the same linear chain of a saccharide (i.e., poly-LacNAc oligosaccharides) or decorating different arms of a multiantennae scaffold, either natural (as in multiantennae N-glycans) or synthetic (of dendrimer or polymer nature). From the lectin perspective, the presence of an individual binding site at every monomer of a multimeric lectin may also have key consequences for the binding event at different levels of complexity.

Graphical abstract: Exploring multivalent carbohydrate–protein interactions by NMR

Article information

Article type
Review Article
Submitted
24 noy 2022
First published
08 fev 2023
This article is Open Access
Creative Commons BY-NC license

Chem. Soc. Rev., 2023,52, 1591-1613

Exploring multivalent carbohydrate–protein interactions by NMR

J. I. Quintana, U. Atxabal, L. Unione, A. Ardá and J. Jiménez-Barbero, Chem. Soc. Rev., 2023, 52, 1591 DOI: 10.1039/D2CS00983H

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