Issue 34, 2023

Selective detection of peroxynitrite using an isatin receptor and a naphthalimide fluorophore

Abstract

Peroxynitrite is a reactive oxygen and nitrogen species that participates in various biological reactions. Therefore, it is important to readily detect and track peroxynitrite in biological systems. Here, a novel turn-on probe encapsulated in PEG DSPE-PEG/HN-I was used to fluorescently detect ONOO rapidly. The encapsulation of HN-I using DSPE-PEG2000 optimizes the sensing performances of the naphthalimide probe and avoids ACQ. Using DSPE-PEG/HN-I to detect changes in the levels of exogenous ONOO in HepG2 cells and endogenous ONOO induced by LPS in RAW 267.4 cells was demonstrated.

Graphical abstract: Selective detection of peroxynitrite using an isatin receptor and a naphthalimide fluorophore

Supplementary files

Article information

Article type
Communication
Submitted
27 noy 2022
Accepted
14 mar 2023
First published
06 apr 2023
This article is Open Access
Creative Commons BY license

Chem. Commun., 2023,59, 5051-5054

Selective detection of peroxynitrite using an isatin receptor and a naphthalimide fluorophore

Y. Wu, H. Han, L. He, L. Li, Y. Zang, J. Li, X. He, Y. Ding, W. Cao and T. D. James, Chem. Commun., 2023, 59, 5051 DOI: 10.1039/D2CC06425A

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