Issue 23, 2022

Pore performance: artificial nanoscale constructs that mimic the biomolecular transport of the nuclear pore complex

Abstract

The nuclear pore complex is a nanoscale assembly that achieves shuttle-cargo transport of biomolecules: a certain cargo molecule can only pass the barrier if it is attached to a shuttle molecule. In this review we summarize the most important efforts aiming to reproduce this feature in artificial settings. This can be achieved by solid state nanopores that have been functionalized with the most important proteins found in the biological system. Alternatively, the nanopores are chemically modified with synthetic polymers. However, only a few studies have demonstrated a shuttle-cargo transport mechanism and due to cargo leakage, the selectivity is not comparable to that of the biological system. Other recent approaches are based on DNA origami, though biomolecule transport has not yet been studied with these. The highest selectivity has been achieved with macroscopic gels, but they are yet to be scaled down to nano-dimensions. It is concluded that although several interesting studies exist, we are still far from achieving selective and efficient artificial shuttle-cargo transport of biomolecules. Besides being of fundamental interest, such a system could be potentially useful in bioanalytical devices.

Graphical abstract: Pore performance: artificial nanoscale constructs that mimic the biomolecular transport of the nuclear pore complex

Article information

Article type
Review Article
Submitted
17 iyn 2022
Accepted
12 sen 2022
First published
13 sen 2022
This article is Open Access
Creative Commons BY license

Nanoscale Adv., 2022,4, 4925-4937

Pore performance: artificial nanoscale constructs that mimic the biomolecular transport of the nuclear pore complex

J. Andersson, J. Svirelis, J. Medin, J. Järlebark, R. Hailes and A. Dahlin, Nanoscale Adv., 2022, 4, 4925 DOI: 10.1039/D2NA00389A

This article is licensed under a Creative Commons Attribution 3.0 Unported Licence. You can use material from this article in other publications without requesting further permissions from the RSC, provided that the correct acknowledgement is given.

Read more about how to correctly acknowledge RSC content.

Social activity

Spotlight

Advertisements