Issue 5, 2022

Pairing cells of different sizes in a microfluidic device for immunological synapse monitoring

Abstract

Analyzing cell–cell interaction is essential to investigate how immune cells function. Elegant designs have been demonstrated to study lymphocytes and their interaction partners. However, these devices have been targeting cells of similar dimensions. T lymphocytes are smaller, more deformable, and more sensitive to pressure than many cells. This work aims to fill the gap of a method for pairing cells with different dimensions. The developed method uses hydrodynamic flow focusing in the z-direction for on-site modulation of effective channel height to capture smaller cells as single cells. Due to immune cells' sensitivity to pressure, the proposed method provides a stable system without any change in flow conditions at the analysis area throughout experiments. Paired live cells have their activities analyzed with calcium imaging at the immunological synapse formed under a controlled environment. The method is demonstrated with primary human T lymphocytes, acute myeloid leukemia (AML) cell lines, and primary AML blasts.

Graphical abstract: Pairing cells of different sizes in a microfluidic device for immunological synapse monitoring

Supplementary files

Article information

Article type
Paper
Submitted
21 dek 2021
Accepted
20 yan 2022
First published
26 yan 2022
This article is Open Access
Creative Commons BY-NC license

Lab Chip, 2022,22, 908-920

Pairing cells of different sizes in a microfluidic device for immunological synapse monitoring

F. A. Shaik, C. Lewuillon, A. Guillemette, B. Ahmadian, C. Brinster, B. Quesnel, D. Collard, Y. Touil, L. Lemonnier and M. C. Tarhan, Lab Chip, 2022, 22, 908 DOI: 10.1039/D1LC01156A

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