Issue 4, 2022

Point-of-care blood coagulation assay enabled by printed circuit board-based digital microfluidics

Abstract

The monitoring of coagulation function has great implications in many clinical settings. However, existing coagulation assays are simplex, sample-consuming, and slow in turnaround, making them less suitable for point-of-care testing. In this work, we developed a novel blood coagulation assay that simultaneously assesses both the tendency of clotting and the stiffness of the resultant clot using printed circuit board (PCB)-based digital microfluidics. A drop of blood was actuated to move back and forth on the PCB electrode array, until the motion winded down as the blood coagulated and became thicker. The velocity tracing and the deformation of the clot were calculated via image analysis to reflect the coagulation progression and the clot stiffness, respectively. We investigated the effect of different hardware and biochemical settings on the assay results. To validate the assay, we performed assays on blood samples with hypo- and hyper-coagulability, and the results confirmed the assay's capability in distinguishing different blood samples. We then examined the correlation between the measured metrics in our assays and standard coagulation assays, namely prothrombin time and fibrinogen level, and the high correlation supported the clinical relevance of our assay. We envision that this method would serve as a powerful point-of-care coagulation testing method.

Graphical abstract: Point-of-care blood coagulation assay enabled by printed circuit board-based digital microfluidics

Supplementary files

Article information

Article type
Paper
Submitted
01 noy 2021
Accepted
13 yan 2022
First published
14 yan 2022

Lab Chip, 2022,22, 709-716

Point-of-care blood coagulation assay enabled by printed circuit board-based digital microfluidics

D. Li, X. Liu, Y. Chai, J. Shan, Y. Xie, Y. Liang, S. Huang, W. Zheng and Z. Li, Lab Chip, 2022, 22, 709 DOI: 10.1039/D1LC00981H

To request permission to reproduce material from this article, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.

Social activity

Spotlight

Advertisements