Issue 8, 2022

Labeling and sequencing nucleic acid modifications using bio-orthogonal tools

Abstract

The bio-orthogonal reaction is a type of reaction that can occur within a cell without interfering with the active components of the cell. Bio-orthogonal reaction techniques have been used to label and track the synthesis, metabolism, and interactions of distinct biomacromolecules in cells. Thus, it is a handy tool for analyzing biological macromolecules within cells. Nucleic acid modifications are widely distributed in DNA and RNA in cells and play a critical role in regulating physiological and pathological cellular activities. Utilizing bio-orthogonal tools to study modified bases is a critical and worthwhile research direction. The development of bio-orthogonal reactions focusing on nucleic acid modifications has enabled the mapping of nucleic acid modifications in DNA and RNA. This review discusses the recent advances in bio-orthogonal labeling and sequencing nucleic acid modifications in DNA and RNA.

Graphical abstract: Labeling and sequencing nucleic acid modifications using bio-orthogonal tools

Article information

Article type
Review Article
Submitted
30 mar 2022
Accepted
20 iyn 2022
First published
22 iyn 2022
This article is Open Access
Creative Commons BY-NC license

RSC Chem. Biol., 2022,3, 994-1007

Labeling and sequencing nucleic acid modifications using bio-orthogonal tools

H. Liu, Y. Wang and X. Zhou, RSC Chem. Biol., 2022, 3, 994 DOI: 10.1039/D2CB00087C

This article is licensed under a Creative Commons Attribution-NonCommercial 3.0 Unported Licence. You can use material from this article in other publications, without requesting further permission from the RSC, provided that the correct acknowledgement is given and it is not used for commercial purposes.

To request permission to reproduce material from this article in a commercial publication, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party commercial publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.

Social activity

Spotlight

Advertisements