Issue 23, 2021

One-pot biocatalytic synthesis of nylon monomers from cyclohexanol using Escherichia coli-based concurrent cascade consortia

Abstract

We developed a multienzyme biocatalytic cascade with high atom efficiency and a self-sufficient redox network for the synthesis of nylon monomers without adding auxiliary enzymes to recycle cofactors. Herein, a cell-based modularization strategy was applied to produce 6-aminohexanoic acid and ε-caprolactam from cyclohexanol. The designed consortia of E. coli containing the respective modules was found to produce >97% of 6-aminohexanoic acid (from 100 mM cyclohexanol) and 28% of caprolactam (from 10 mM cyclohexanol) with 2-fold excess of propylamine in a one-pot reaction. The use of cheaper substrates and co-substrates makes it an economically beneficial approach for the production of nylon precursors.

Graphical abstract: One-pot biocatalytic synthesis of nylon monomers from cyclohexanol using Escherichia coli-based concurrent cascade consortia

Supplementary files

Article information

Article type
Communication
Submitted
23 avq 2021
Accepted
04 noy 2021
First published
04 noy 2021

Green Chem., 2021,23, 9447-9453

One-pot biocatalytic synthesis of nylon monomers from cyclohexanol using Escherichia coli-based concurrent cascade consortia

S. Sarak, T. P. Khobragade, H. Jeon, A. D. Pagar, P. Giri, S. Lee and H. Yun, Green Chem., 2021, 23, 9447 DOI: 10.1039/D1GC03056F

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