Issue 77, 2021

A novel 18F-labeled clickable substrate for targeted imaging of SNAP-tag expressing cells by PET in vivo

Abstract

Bioorthogonal covalent labeling with self-labeling enzymes like SNAP-tag bears a high potential for specific targeting of cells for imaging in vitro and also in vivo. To this end, fluorescent SNAP substrates have been established and used in microscopy and fluorescence imaging while radioactive substrates for the highly sensitive and whole-body positron emission tomography (PET) have been lacking. Here, we show for the first time successful and high-contrast PET imaging of subcutaneous SNAP-tag expressing tumor xenografts by bioorthogonal covalent targeting with a novel 18F-based radioligand in vivo.

Graphical abstract: A novel 18F-labeled clickable substrate for targeted imaging of SNAP-tag expressing cells by PET in vivo

Supplementary files

Article information

Article type
Communication
Submitted
16 iyl 2021
Accepted
31 avq 2021
First published
31 avq 2021
This article is Open Access
Creative Commons BY-NC license

Chem. Commun., 2021,57, 9850-9853

A novel 18F-labeled clickable substrate for targeted imaging of SNAP-tag expressing cells by PET in vivo

D. A. Depke, C. P. Konken, L. Rösner, S. Hermann, M. Schäfers and A. Rentmeister, Chem. Commun., 2021, 57, 9850 DOI: 10.1039/D1CC03871K

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