Issue 47, 2020

Development and application of ubiquitin-based chemical probes

Abstract

Protein ubiquitination regulates almost every process in eukaryotic cells. The study of the many enzymes involved in the ubiquitination system and the development of ubiquitination-associated therapeutics are important areas of current research. Synthetic tools such as ubiquitin-based chemical probes have been making an increasing contribution to deciphering various biochemical components involved in ubiquitin conjugation, recruitment, signaling, and deconjugation. In the present minireview, we summarize the progress of ubiquitin-based chemical probes with an emphasis on their various structures and chemical synthesis. We discuss the utility of the ubiquitin-based chemical probes for discovering and profiling ubiquitin-dependent signaling systems, as well as the monitoring and visualization of ubiquitin-related enzymatic machinery. We also show how the probes can serve to elucidate the molecular mechanism of recognition and catalysis. Collectively, the development and application of ubiquitin-based chemical probes emphasizes the importance and utility of chemical protein synthesis in modern chemical biology.

Graphical abstract: Development and application of ubiquitin-based chemical probes

Article information

Article type
Minireview
Submitted
15 iyn 2020
Accepted
18 iyl 2020
First published
04 avq 2020
This article is Open Access

All publication charges for this article have been paid for by the Royal Society of Chemistry
Creative Commons BY license

Chem. Sci., 2020,11, 12633-12646

Development and application of ubiquitin-based chemical probes

X. Sui, Y. Wang, Y. Du, L. Liang, Q. Zheng, Y. Li and L. Liu, Chem. Sci., 2020, 11, 12633 DOI: 10.1039/D0SC03295F

This article is licensed under a Creative Commons Attribution 3.0 Unported Licence. You can use material from this article in other publications without requesting further permissions from the RSC, provided that the correct acknowledgement is given.

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