Issue 9, 2020

Methylation dependent gold adsorption behaviour identifies cancer derived extracellular vesicular DNA

Abstract

Extracellular vesicles (EV) play a major role in intercellular communication by transmitting cellular materials (e.g. protein, RNA) among distant cells. Recent evidence suggests that they could also contribute to carrying DNA which could inform on the mutational status of the parent tumour DNA. Thus, the fundamental analysis of evDNA could open a better understanding of tumour metastasis and provide new pathways for noninvasive detection and monitoring of cancer. To explore the potential of evDNA for diagnostics, the isolation of pure evDNA from body fluids free of cfDNA contamination is crucial. Herein, we use a liposome based model system to develop an improved evDNA isolation protocol free from cfDNA contamination and evaluate the methylation dependent physicochemical properties of evDNA to develop a simple test for detecting cancer evDNA. Using a highly sensitive multiplex microelectrode device, we demonstrate that serum-evDNA derived from cancer patients show different solution and surface based properties than normal evDNA due to their different methylation landscape (i.e. methylscape). This microdevice allows simultaneous analysis of multiple samples in a single platform from as low as 500 pg μL−1 of evDNA.

Graphical abstract: Methylation dependent gold adsorption behaviour identifies cancer derived extracellular vesicular DNA

Supplementary files

Article information

Article type
Communication
Submitted
09 iyl 2019
Accepted
03 iyn 2020
First published
04 iyn 2020

Nanoscale Horiz., 2020,5, 1317-1323

Methylation dependent gold adsorption behaviour identifies cancer derived extracellular vesicular DNA

A. A. I. Sina, T. Lin, R. Vaidyanathan, Z. Wang, S. Dey, J. Wang, A. Behren, A. Wuethrich, L. G. Carrascosa and M. Trau, Nanoscale Horiz., 2020, 5, 1317 DOI: 10.1039/D0NH00258E

To request permission to reproduce material from this article, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.

Social activity

Spotlight

Advertisements