Issue 16, 2020

Enhancement of inflection point focusing and rare-cell separations from untreated whole blood

Abstract

Inertial microfluidic systems have been widely used for particle or cell separation applications, especially for rare-cell enrichment and separation from blood due to the high throughput and simplicity of the systems. However, most of the separation techniques using inertial microfluidic systems require dilution of blood samples or RBC lysis to achieve a high separation efficiency, which can adversely affect the throughput and/or analysis of the collected sample. We developed a cell separation technique compatible with untreated whole blood by inflection point focusing, that is, an inertial focusing phenomenon toward inflection points of the velocity profile. We generated a strong shear-gradient lift force by varying the velocity profile in a channel cross-section with the combined effect of the channel geometry and the co-flows of two liquids with different viscosities. The natural viscosity of blood leads to a lagging flow region in the middle of the channel where larger particles and cells can be extracted to the side flow region, which enables a highly efficient separation scheme with an unprecedented high throughput.

Graphical abstract: Enhancement of inflection point focusing and rare-cell separations from untreated whole blood

Supplementary files

Article information

Article type
Paper
Submitted
26 mar 2020
Accepted
25 iyn 2020
First published
29 iyn 2020

Lab Chip, 2020,20, 2861-2871

Enhancement of inflection point focusing and rare-cell separations from untreated whole blood

D. Lee, Y. Choi and W. Lee, Lab Chip, 2020, 20, 2861 DOI: 10.1039/D0LC00309C

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