Issue 9, 2019

Rapid analytical characterization of high-throughput chemistry screens utilizing desorption electrospray ionization mass spectrometry

Abstract

A detailed overview of a newly developed method for the analysis and processing of chemical library screens is reported. Integration of a DESI 2D source in a pharmaceutical drug discovery setting is accomplished by utilizing this platform on multiple reaction screens sets including Buchwald and Suzuki couplings, fluorination chemistry, and monomer reactions. Custom software developed for the DESI data analysis enables automated data processing, review and favorable comparisons with UPLC results. The new method represents a 25-fold reduction in reaction screening sets analysis time over conventional state of the art UPLC/MS, with the ability to scale as reaction sets increase in size. In addition to all of the speed and throughput improvements, DESI is very efficient, requiring only 500 nL of sample volume for testing and uses only a few mL of solvent a day.

Graphical abstract: Rapid analytical characterization of high-throughput chemistry screens utilizing desorption electrospray ionization mass spectrometry

Supplementary files

Article information

Article type
Paper
Submitted
31 yan 2019
Accepted
18 apr 2019
First published
18 apr 2019

React. Chem. Eng., 2019,4, 1589-1594

Rapid analytical characterization of high-throughput chemistry screens utilizing desorption electrospray ionization mass spectrometry

J. W. Sawicki, A. R. Bogdan, P. A. Searle, N. Talaty and S. W. Djuric, React. Chem. Eng., 2019, 4, 1589 DOI: 10.1039/C9RE00054B

To request permission to reproduce material from this article, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.

Social activity

Spotlight

Advertisements