Issue 2, 2019

Detection of microRNA biomarkers via inhibition of DNA-mediated liposome fusion

Abstract

We report the specific and sensitive detection of microRNA using an inverse DNA-mediated liposome fusion assay. This assay is homogeneous, and does not require washing, separation, or enzyme-associated amplification steps. By fine-tuning the surface functionalisation of the liposomes, liposome concentration, and assay temperature, we demonstrated a sub-nanomolar limit of detection for the target.

Graphical abstract: Detection of microRNA biomarkers via inhibition of DNA-mediated liposome fusion

Supplementary files

Article information

Article type
Communication
Submitted
08 noy 2018
Accepted
14 noy 2018
First published
21 noy 2018
This article is Open Access
Creative Commons BY license

Nanoscale Adv., 2019,1, 532-536

Detection of microRNA biomarkers via inhibition of DNA-mediated liposome fusion

C. Jumeaux, E. Kim, P. D. Howes, H. Kim, R. Chandrawati and M. M. Stevens, Nanoscale Adv., 2019, 1, 532 DOI: 10.1039/C8NA00331A

This article is licensed under a Creative Commons Attribution 3.0 Unported Licence. You can use material from this article in other publications without requesting further permissions from the RSC, provided that the correct acknowledgement is given.

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