Issue 5, 2019

Hydroxynitrile lyases covalently immobilized in continuous flow microreactors

Abstract

Enzymes are supreme catalysts when it comes to high enantiopurities and their immobilization will pave the way for continuous operation. In this context, we show the covalent immobilization of hydroxynitrile lyases HbHNL (from Hevea brasiliensis) and MeHNL (from Manihot esculenta) in a siliceous monolithic microreactor for continuous operation. A thorough characterization of the immobilized HNLs on mesoporous silicates indicated the conditions essential for a successful immobilization. Their application in a continuous flow system enabled a remarkably fast (3.2 min) production of chiral cyanohydrins with high conversion (97%) and high ee (98%) using minimal enzyme loading (STY = 71 g L−1 h−1 mgprotein−1). MeHNL showed increased operational stability, possibly due to a structural difference. The continuous flow microreactor outperformed batch systems, demonstrating the advantage of the mesoporous/macroporous environment for the expression of enzyme activity and the favorable characteristics of the microreactor. Overall, the system shows great potential for future industrial application of biocatalytic asymmetric syntheses.

Graphical abstract: Hydroxynitrile lyases covalently immobilized in continuous flow microreactors

Supplementary files

Article information

Article type
Paper
Submitted
23 okt 2018
Accepted
15 yan 2019
First published
11 fev 2019
This article is Open Access
Creative Commons BY-NC license

Catal. Sci. Technol., 2019,9, 1189-1200

Hydroxynitrile lyases covalently immobilized in continuous flow microreactors

M. P. van der Helm, P. Bracco, H. Busch, K. Szymańska, A. B. Jarzębski and U. Hanefeld, Catal. Sci. Technol., 2019, 9, 1189 DOI: 10.1039/C8CY02192A

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