Issue 4, 2018

A label-free electrochemical platform for the highly sensitive detection of hepatitis B virus DNA using graphene quantum dots

Abstract

Based on the strong interaction between single-stranded DNA and graphene material, we have designed a simple but smart electrochemical platform to detect HBV-DNA by using a graphene quantum dot (GQD) modified glassy carbon electrode coupled with specific sequence DNA molecules as probes. The probe DNA is designed to be complementary to the HBV-DNA, when the probe DNA is strongly bound to the surface of the GQD modified electrode the transfer of an electron from the electrode to the electrochemically active species K3[Fe(CN)6] will become difficult. Nevertheless, if the target HBV-DNA is found in the test solution, the probe DNA will bind with the target HBV-DNA instead of GQDs. As a result, the obtained peak currents of K3[Fe(CN)6] will have a different degree of increase with the different concentrations of the target HBV-DNA. In particular, the proposed sensor exhibits high sensitivity with a detection limit of 1 nM, and the linear detection range is from 10 nM to 500 nM. Additionally, the sensor could be used in detecting other probe DNA, which may have potential applications in the future.

Graphical abstract: A label-free electrochemical platform for the highly sensitive detection of hepatitis B virus DNA using graphene quantum dots

Article information

Article type
Paper
Submitted
30 okt 2017
Accepted
23 dek 2017
First published
08 yan 2018
This article is Open Access
Creative Commons BY license

RSC Adv., 2018,8, 1820-1825

A label-free electrochemical platform for the highly sensitive detection of hepatitis B virus DNA using graphene quantum dots

Q. Xiang, J. Huang, H. Huang, W. Mao and Z. Ye, RSC Adv., 2018, 8, 1820 DOI: 10.1039/C7RA11945C

This article is licensed under a Creative Commons Attribution 3.0 Unported Licence. You can use material from this article in other publications without requesting further permissions from the RSC, provided that the correct acknowledgement is given.

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