Issue 21, 2018

Biomolecular recognition on nanowire surfaces modified by the self-assembled monolayer

Abstract

Molecular recognition is one of the key factors in designing biosensors due to which nanowires functionalized with molecular recognition have attracted a lot of attention as promising candidates for nanostructures embedded in biosensors. However, the difficulty in real-world applications with analytical specificity is that molecular recognition on nanowires mainly depends on antibody modification with multistep modification procedures. Furthermore, the antibody modification suffers from nonspecific adsorption of undesired proteins in body fluid on the nanowires, which causes false responses and lowers sensitivity. Herein, we propose biomolecular recognition using surface-modified nanowires via thiolated 2-methacryloxyethyl phosphorylcholine (MPC-SH). MPC-SH enables self-assembled monolayer (SAM) modification, which contributes to the reduction of nonspecific adsorption of biomolecules onto the nanowires, and the specific capture of a target protein is attained in the presence of calcium ions. Our concept demonstrates the recognition of the biomarker protein on nanowire surfaces modified by MPC-SH SAM with a single step modification procedure.

Graphical abstract: Biomolecular recognition on nanowire surfaces modified by the self-assembled monolayer

Supplementary files

Article information

Article type
Communication
Submitted
27 apr 2018
Accepted
16 iyl 2018
First published
28 sen 2018
This article is Open Access
Creative Commons BY-NC license

Lab Chip, 2018,18, 3225-3229

Biomolecular recognition on nanowire surfaces modified by the self-assembled monolayer

T. Shimada, T. Yasui, A. Yokoyama, T. Goda, M. Hara, T. Yanagida, N. Kaji, M. Kanai, K. Nagashima, Y. Miyahara, T. Kawai and Y. Baba, Lab Chip, 2018, 18, 3225 DOI: 10.1039/C8LC00438B

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