Issue 18, 2018

Continuous removal of small nonviable suspended mammalian cells and debris from bioreactors using inertial microfluidics

Abstract

Removing nonviable cells from a cell suspension is crucial in biotechnology and biomanufacturing. Label-free microfluidic cell separation devices based on dielectrophoresis, acoustophoresis, and deterministic lateral displacement are used to remove nonviable cells. However, their volumetric throughputs and test cell concentrations are generally too low to be useful in typical bioreactors in biomanufacturing. In this study, we demonstrate the efficient removal of small (<10 μm) nonviable cells from bioreactors while maintaining viable cells using inertial microfluidic cell sorting devices and characterize their performance. Despite the size overlap between viable and nonviable cell populations, the devices demonstrated 3.5–28.0% dead cell removal efficiency with 88.3–83.6% removal purity as well as 97.8–99.8% live cell retention efficiency at 4 million cells per mL with 80% viability. Cascaded and parallel configurations increased the cell concentration capacity (10 million cells per mL) and volumetric throughput (6–8 mL min−1). The system can be used for the removal of small nonviable cells from a cell suspension during continuous perfusion cell culture operations.

Graphical abstract: Continuous removal of small nonviable suspended mammalian cells and debris from bioreactors using inertial microfluidics

Supplementary files

Article information

Article type
Paper
Submitted
08 mar 2018
Accepted
31 iyl 2018
First published
31 iyl 2018
This article is Open Access
Creative Commons BY-NC license

Lab Chip, 2018,18, 2826-2837

Continuous removal of small nonviable suspended mammalian cells and debris from bioreactors using inertial microfluidics

T. Kwon, R. Yao, J. P. Hamel and J. Han, Lab Chip, 2018, 18, 2826 DOI: 10.1039/C8LC00250A

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