A novel colorimetric assay for α-methylacyl-CoA racemase 1A (AMACR; P504S) utilizing the elimination of 2,4-dinitrophenolate

Maksims Yevglevskis; Guat L. Lee; Amit Nathubhai; Yoana D. Petrova; Tony D. James; Michael D. Threadgill; Timothy J. Woodman; Matthew D. Lloyd

doi:10.1039/C7CC00476A

You do not have JavaScript enabled. Please enable JavaScript to access the full features of the site or access our non-JavaScript page.

A novel colorimetric assay for α-methylacyl-CoA racemase 1A (AMACR; P504S) utilizing the elimination of 2,4-dinitrophenolate†

Maksims Yevglevskis,^a Guat L. Lee,^a Amit Nathubhai,^a Yoana D. Petrova,^a Tony D. James,

^b Michael D. Threadgill,^a Timothy J. Woodman^a and Matthew D. Lloyd

*^a

Author affiliations

* Corresponding authors

^a Drug & Target Development, Department of Pharmacy & Pharmacology, University of Bath, Claverton Down, Bath BA2 7AY, UK
E-mail: M.D.Lloyd@bath.ac.uk
Fax: +-44-1225-386114
Tel: +-44-1225-386786

^b Department of Chemistry, University of Bath, Claverton Down, Bath BA2 7AY, UK

Abstract

α-Methylacyl-CoA racemase (AMACR; P504S) regulates branched-chain fatty acid degradation, activates Ibuprofen and is a recognised cancer drug target. A novel, facile colorimetric assay was developed based on elimination of 2,4-dinitrophenolate. The assay was used to test 5 known inhibitors, determining IC₅₀ and K_i values, reversibility and characterizing irreversible inhibition.

This article is part of the themed collection: Chemosensors and Molecular Logic

Download options Please wait...

Supplementary files

Article information

DOI: https://doi.org/10.1039/C7CC00476A
Article type: Communication
Submitted: 18 yan 2017
Accepted: 17 mar 2017
First published: 17 mar 2017
This article is Open Access

Download Citation

Chem. Commun., 2017,53, 5087-5090

Permissions

Request permissions

A novel colorimetric assay for α-methylacyl-CoA racemase 1A (AMACR; P504S) utilizing the elimination of 2,4-dinitrophenolate

M. Yevglevskis, G. L. Lee, A. Nathubhai, Y. D. Petrova, T. D. James, M. D. Threadgill, T. J. Woodman and M. D. Lloyd, Chem. Commun., 2017, 53, 5087 DOI: 10.1039/C7CC00476A

This article is licensed under a Creative Commons Attribution 3.0 Unported Licence. You can use material from this article in other publications without requesting further permissions from the RSC, provided that the correct acknowledgement is given.

Social activity

Fetching data from CrossRef.
This may take some time to load.

Chemical Communications

A novel colorimetric assay for α-methylacyl-CoA racemase 1A (AMACR; P504S) utilizing the elimination of 2,4-dinitrophenolate†

Abstract

Supplementary files

Article information

Download Citation

Permissions

A novel colorimetric assay for α-methylacyl-CoA racemase 1A (AMACR; P504S) utilizing the elimination of 2,4-dinitrophenolate

Social activity

Search articles by author

Spotlight

Advertisements