Issue 91, 2016

Dual drug-loaded halloysite hybrid-based glycocluster for sustained release of hydrophobic molecules

Abstract

A dual drug-loaded HNT–CD glycocluster delivery system based on halloysite nanotubes and carbohydrate functionalized cyclodextrin was developed by a green protocol using solvent-free microwave irradiation. The nanohybrid was employed for concurrent load and release of silibinin and curcumin. The new delivery system was characterized by means of TGA, FT-IR spectroscopy, SEM and DLS. These techniques confirm the successful loading of the two drugs in the system. SEM and DLS measurements highlighted that the nanomaterial preserves a tubular structure with an average hydrodynamic radius of ca. 200 nm. The release of the drugs from the HNT glycocluster was investigated by means of UV-vis spectroscopy at two different pH values simulanting the typical physiological conditions of either gastric or intestinal fluids. Enzyme-linked lectin assays (ELLA) demonstrated that highly mannoside–cyclodextrins HNT entities display high affinity towards mannose selective ConA lectin. Biological assays showed that the new drug delivery system exhibits anti-proliferative activity against the investigated cell lines. Fluorescence microscopy confirmed ELLA results and it showed a high propensity of this drug delivery system to cross cell membranes and to penetrate into the cell nucleus. The results revealed that the synthesized multicavity system is a material of suitable size and nanoarchitecture to transport drugs into living cells.

Graphical abstract: Dual drug-loaded halloysite hybrid-based glycocluster for sustained release of hydrophobic molecules

Supplementary files

Article information

Article type
Paper
Submitted
06 iyn 2016
Accepted
08 sen 2016
First published
08 sen 2016

RSC Adv., 2016,6, 87935-87944

Dual drug-loaded halloysite hybrid-based glycocluster for sustained release of hydrophobic molecules

M. Massaro, S. Riela, C. Baiamonte, J. L. J. Blanco, C. Giordano, P. Lo Meo, S. Milioto, R. Noto, F. Parisi, G. Pizzolanti and G. Lazzara, RSC Adv., 2016, 6, 87935 DOI: 10.1039/C6RA14657K

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