Issue 80, 2016

A biocompatible serine functionalized nanostructured zirconia based biosensing platform for non-invasive oral cancer detection

Abstract

We report results of studies relating to the fabrication of nontoxic, biocompatible serine functionalized nanostructured zirconia (serine/nZrO2) for oral cancer detection. The hydrothermally synthesized ZrO2 nanoparticles (∼4.5 nm) have been characterized using X-ray diffraction (XRD) and transmission electron microscopy (TEM). The cytotoxicity studies of serine/nZrO2 carried out using MTT assay on the HEK 293 human cell line reveal biocompatibility of the serine/nZrO2. The serine functionalized nZrO2 are electrophoretically deposited onto a hydrolysed indium tin oxide (ITO) coated glass electrode (serine/nZrO2/ITO). Furthermore, monoclonal antibodies (anti-CYFRA-21-1) were covalently immobilized onto the serine/nZrO2/ITO electrode and BSA was been used for blocking of the non-specific binding sites. The fabricated BSA/anti-CYFRA-21-1/serine/nZrO2/ITO immunoelectrode was used for label free, non-invasive and efficient detection of an oral cancer biomarker (CYFRA-21-1). The electrochemical response studies of this immunoelectrode exihibited a wider linear detection range (0.01–29 ng mL−1) with a remarkable detection limit (0.01 ng mL−1) and a quick response time (6 min) for detection of the CYFRA-21-1 antigen. The results of validation studies conducted on the fabricated immunoelectrode (BSA/anti-CYFRA-21-1/serine/nZrO2/ITO) using saliva samples of oral cancer patients indicate a good agreement with the results obtained via enzyme linked immunosorbent assay (ELISA).

Graphical abstract: A biocompatible serine functionalized nanostructured zirconia based biosensing platform for non-invasive oral cancer detection

Supplementary files

Article information

Article type
Paper
Submitted
21 mar 2016
Accepted
29 iyl 2016
First published
08 avq 2016

RSC Adv., 2016,6, 77037-77046

A biocompatible serine functionalized nanostructured zirconia based biosensing platform for non-invasive oral cancer detection

S. Kumar, J. G. Sharma, S. Maji and B. D. Malhotra, RSC Adv., 2016, 6, 77037 DOI: 10.1039/C6RA07392A

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